FastQCFastQC Report
Tue 14 Mar 2017
HYMN3BGXY_n01_9s-18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYMN3BGXY_n01_9s-18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17688770
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC194900.11018290135492745No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG41600.040.7156415
CACGTCT51050.033.6515814
CAGTCAC49700.032.34740427
CTCGTAT24350.031.8785442
ACACGTC54500.031.58463913
CACCGAT55800.028.62420331
TCGTATG23000.028.35028543
AGTCACC59750.027.1950928
GTCTGAA65000.026.74763317
TATGCCG29600.026.34207546
TGTTACC38900.025.71699713
TATCTCG35650.025.44963839
CTTAAAC48800.025.1818121
CGATGTA63950.024.76018134
TTTTTCC90050.024.6481321
TTACCTC40450.024.5610615
ACCTATA49450.023.3728566
CGCTATG50000.023.04531929
AGCACAC76750.022.92252310
CTAATGC43000.022.86489121
Produced by FastQC (version 0.11.2)