FastQCFastQC Report
Tue 14 Mar 2017
HYMN3BGXY_n01_9s-13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYMN3BGXY_n01_9s-13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14910571
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG224630.15065150757808No Hit
CTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACGGCGCCGGAGGCACGACCCGGCCAGTTAAGGCCAGGAGCGC177410.11898270026010406No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG169200.11347653956377661No Hit
CTTTGAGCACTCTAATTTCTTCAAAGTAACGGCGCCGGAGGCACGACCCGGCCAGTTAAGGCCAGGAGCGCATCG149340.10015713013270922No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG29050.038.94985615
ACACGTC34000.033.38045513
CACGTCT34200.033.28611414
CAGTCAC36950.028.10305627
CTCGTAT16350.026.16433742
CTATAAG63000.026.1214246
CGCTATG36650.024.28550729
CTTAAAC32750.024.2431471
TATAAGC67900.024.23644847
ACATCCG70200.023.9385755
GTCTGAA47900.023.69353317
AGTCACC43250.023.61052328
GTCGCTA38400.022.81938727
TCGTATG16250.022.50430543
ACCTATA35300.022.1862896
GCGGAGT76700.021.85894838
GTCCCTA46150.021.7515018
CGGAGTC77850.021.49173539
GAGTCCT79350.021.25943641
CCCTGGT78300.021.19248467
Produced by FastQC (version 0.11.2)