FastQCFastQC Report
Tue 14 Mar 2017
HYMN3BGXY_n01_9s-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYMN3BGXY_n01_9s-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15506505
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG190520.12286456554845854No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC176200.11362973152235142No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT27200.044.13941242
ACGTCTG31050.043.6625715
AGTCACG28850.043.40718528
TCGTATG28300.042.42429443
TATGCCG30350.040.1281446
CACGTCT35950.037.2315614
GTCACGC36300.034.87891829
CAGTCAC37100.034.12648427
GCCAATA38300.032.7861834
ACACGTC43000.032.25046513
TATCTCG37750.031.98468439
GTATGCC39500.031.00682345
CACGCCA40950.030.41185231
TCACGCC43100.030.0958930
CGCCAAT41650.029.90043833
CGTATGC40650.029.53533244
ATGCCGT42700.029.32988447
TGTTACC33050.028.39068213
CCAATAT46900.026.77404235
CTTAAAC36550.026.7265051
Produced by FastQC (version 0.11.2)