FastQCFastQC Report
Tue 14 Mar 2017
HYMN3BGXY_n01_4w-47.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYMN3BGXY_n01_4w-47.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13374787
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG25650.032.81519315
ACACGTC26750.031.8525413
CACGTCT30200.028.32808714
TTACCTC24150.025.85436815
CTTAAAC37000.025.7482591
TCGTATG17050.025.49582343
TGTTACC26800.023.81265613
GTCGCTA37000.023.775927
CGCTATG36750.023.74962629
CAGTCAC32600.023.59857227
ACCTATA40400.023.14276
CTCGTAT14250.022.75783542
GTCGTAT7500.022.07994342
TATGCCG21500.021.5021946
GGTAACT41550.021.42272869
TAGTTGT31900.021.4113379
CTAATGC28200.021.16349421
CGTATGC21200.021.15644
TATAACC43950.021.1136179
TCTAATG29400.021.00347920
Produced by FastQC (version 0.11.2)