FastQCFastQC Report
Tue 14 Mar 2017
HYMN3BGXY_n01_4w-39.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYMN3BGXY_n01_4w-39.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14825570
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG216120.14577517087032743No Hit
CTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACGGCGCCGGAGGCACGACCCGGCCAGTTAAGGCCAGGAGCGC150110.10125074449076832No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG25300.040.35931415
TCGTATG20650.040.2632743
CTCGTAT19600.038.01979442
TATGCCG24050.035.0024646
CACGTCT30900.033.15641814
ACACGTC35550.029.4986513
TGTTACC30300.029.48664313
CAGTCAC32700.029.11771627
ACTCAAT33900.028.49329435
CTATAAG64300.026.66669846
CGTATGC32200.026.142744
TTACCTC33900.025.64327215
GTATGCC34800.025.37898845
CTAATGC34850.024.84617821
ACATCCG68550.024.81702655
GTCTGAA41700.024.7347617
TATAAGC68950.024.71868147
CGCTATG38250.024.71249429
TTTTTCC68250.024.6808871
TCTAGTT41050.024.5380087
Produced by FastQC (version 0.11.2)