FastQCFastQC Report
Tue 14 Mar 2017
HYMN3BGXY_n01_4w-38.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYMN3BGXY_n01_4w-38.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15687815
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG216050.13771835019727094No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG171670.10942887840021062No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG29150.042.48906743
ACGTCTG32900.041.7315215
CTCGTAT26850.040.2171942
TATGCCG31450.039.49259646
CACGTCT38400.036.2031814
CAGTCAC38350.033.46455827
ACACGTC42000.033.2643213
GTATGCC40650.030.63863445
CTAATCT35800.030.45024337
CGTATGC40950.030.41417544
ATGCCGT43300.028.92361347
TAATCTC40250.027.16931738
CGCTATG36500.027.032529
AGTCACC47600.026.81659728
GTCTGAA53100.026.4406917
CTATAAG65050.025.458246
GTCGCTA38700.025.31715227
CTTAAAC36550.025.216741
ACATCCG71500.023.45465955
ACCTATA39050.023.4126366
Produced by FastQC (version 0.11.2)