FastQCFastQC Report
Tue 14 Mar 2017
HYMN3BGXY_n01_4w-35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYMN3BGXY_n01_4w-35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15143384
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC193880.1280295078035398No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA157160.10378129485457148TruSeq Adapter, Index 25 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT31900.047.26289444
TCGTATG35550.044.73888445
ACGTCTG40200.043.0774415
GTCACAC39000.041.6647429
TATGCCG39350.039.8060748
CGTATGC43200.036.65713546
CACGTCT48200.035.99922614
TATCTCG42750.035.83031541
AGTCACA45550.035.52183528
TCACACT47150.034.8275830
ACACGTC50150.034.59934213
CAGTCAC47700.033.8484227
ACTGATA49450.032.64882334
GTATGCC50500.031.63145647
ATGCCGT50200.031.40916649
TATATCT49550.031.19091239
GTCTGAA59300.029.55141817
TTTTTCC85950.028.9575211
ATATCTC53400.028.87733540
TGTTACC40450.027.37519813
Produced by FastQC (version 0.11.2)