FastQCFastQC Report
Tue 14 Mar 2017
HYMN3BGXY_n01_4w-34.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYMN3BGXY_n01_4w-34.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15162019
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG212120.13990221223176147No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG163840.1080594873281718No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC160540.10588299618935974No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT29100.043.15404544
ACGTCTG37050.043.10935215
TCGTATG30100.042.86609645
TATGCCG33800.038.78862448
TATCTCG35500.036.4414841
GTCACAT42350.034.94699529
CACGTCT46800.034.4966814
CAGTCAC43600.034.02408627
ACACGTC48600.033.71589313
AGTCACA44350.033.52660828
CGTATGC41900.031.12388246
GTATGCC42700.030.94525347
ACATTCC49250.030.04928232
GTCTGAA55600.029.16038317
ATGCCGT44600.028.62294849
TGTTACC33500.028.21519713
CACATTC52700.028.14775531
TTATCTC48150.027.65557140
TTACCTC35400.026.5060115
TAGTTGT38750.025.104029
Produced by FastQC (version 0.11.2)