FastQCFastQC Report
Tue 14 Mar 2017
HYMN3BGXY_n01_4w-32.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYMN3BGXY_n01_4w-32.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16859730
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG42800.041.3479415
CACGTCT52250.034.39777814
AGTCACA49050.034.39356228
ACACGTC52600.034.03772713
CAGTCAC53550.031.8253727
GTCACAT62750.026.71943729
GTCTGAA67950.026.44970117
CACATGA65100.026.28359831
CACACGT69150.025.74165712
ACATGAG67100.025.60300432
CGCTATG38650.023.1182529
ATCGGAA76700.022.715722
CCAGTCA76400.022.12622826
GTCGCTA40400.021.60454627
CTTAAAC36200.021.3582551
TGTTACC35900.021.3322913
TTTTTCC82350.021.2924881
CTCCAGT85900.021.20535324
TCTGAAC85900.021.20390518
TCCAGTC84350.021.06332225
Produced by FastQC (version 0.11.2)