FastQCFastQC Report
Tue 14 Mar 2017
HYMN3BGXY_n01_4w-25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYMN3BGXY_n01_4w-25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13094220
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC194920.1488595731551784No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT26700.042.8979742
ACGTCTG31250.041.39643515
TCGTATG29000.040.68580643
TATGCCG30300.038.94080746
ACACGTC38400.034.22712713
CACGTCT38700.033.69438614
CGTATGC37550.031.32991844
GTATGCC39900.029.5712545
CAGTCAC41200.029.30706827
TGTTACC37950.027.90636613
ATGCCGT43250.027.6003647
TTTTTCC86200.026.7900941
TTACCTC39400.026.70455215
CACACGT49750.026.27984612
GTCTGAA51200.026.07490217
CGCTATG31500.025.29878629
TCTAGTT46700.025.1153227
AGTCACC48050.024.91347728
TTTTCCA96000.024.6903292
CTAATGC42450.024.05501621
Produced by FastQC (version 0.11.2)