FastQCFastQC Report
Tue 14 Mar 2017
HYMN3BGXY_n01_4w-22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYMN3BGXY_n01_4w-22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13461153
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC161130.11969999895254144No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG29650.043.3966115
TCGTATG25150.042.38698243
CTCGTAT25300.041.3159742
ACGATAT31650.037.4943735
TATGCCG29550.035.84338446
CACGTCT36750.035.66943714
ACACGTC38500.035.12330613
CAGTCAC34100.034.80204427
CCACGAT35350.034.25277733
TATCTCG34050.031.91352539
CGATATC36950.030.8092336
CGTATGC37000.028.90503944
TGTTACC30300.027.89288913
CACGATA44500.027.44266134
GTATGCC39350.027.3543745
GTCTGAA48400.027.08371717
AGTCACC44900.026.8152228
ATGCCGT41050.025.97037747
CTTAAAC38200.025.6653841
ACCACGA47650.025.5559232
Produced by FastQC (version 0.11.2)