FastQCFastQC Report
Tue 14 Mar 2017
HYMN3BGXY_n01_4s-47.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYMN3BGXY_n01_4s-47.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15483337
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG217770.1406479753040317No Hit
CTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACGGCGCCGGAGGCACGACCCGGCCAGTTAAGGCCAGGAGCGC158070.10209039562983095No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG27250.034.68622215
AGTCACG26800.031.66751328
CTCGTAT19350.031.37965442
CACGTCT31900.029.73822214
TCGTATG21150.027.89389643
ACACGTC34450.027.23649813
CTATAAG65650.026.80200846
CAGTCAC33400.025.92640527
TATGCCG24000.025.1569946
TATAAGC71150.024.87588547
CTTAAAC35900.024.7119061
ACATCCG72050.024.47254455
CGCTATG37800.023.54717329
GTCCCTA44400.023.3081868
CGTATGC25750.022.9109144
ACCTATA38400.022.2816186
GGTAGCA39500.022.183134
ACATGCG36500.021.9291294
CATGCGT35600.021.9020465
CGGAGTC81150.021.72290839
Produced by FastQC (version 0.11.2)