FastQCFastQC Report
Tue 14 Mar 2017
HYMN3BGXY_n01_4s-39.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYMN3BGXY_n01_4s-39.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15492368
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT29950.042.1613144
ACGTCTG34050.041.13107315
TCGTATG33150.039.65256545
CACGAGT34650.038.03066331
TATGCCG35650.036.38940448
AGTCACG37500.035.7861128
ACACGTC40700.034.91917413
CACGTCT41000.034.4953914
TATCTCG37150.033.9875541
ACGAGTG40700.032.88578832
CGTATGC41550.031.719546
TCACGAG42900.031.3610730
CAGTCAC43600.030.85856227
GTATGCC44600.029.47298647
GTCACGA45850.029.26833729
GTCTGAA52850.026.89122217
ATGCCGT49050.026.589149
CGCTATG50100.025.75263429
GTCGCTA51000.025.5693227
AGTGGAT55750.024.25635
Produced by FastQC (version 0.11.2)