FastQCFastQC Report
Tue 14 Mar 2017
HYMN3BGXY_n01_4s-38.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYMN3BGXY_n01_4s-38.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14011287
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG153540.10958308112595223No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG21650.048.2802433
ACGTCTG27550.042.07214415
ACGTTTC26400.039.72398832
GTCACGT26750.039.33474329
CACGTTT27950.037.39789231
TTTCGGA28450.037.34744635
TTCGGAA29200.036.15189436
CACGTCT33150.035.06899614
AGTCACG30050.034.67082628
ACACGTC36150.032.540313
TCGGAAT32500.032.16264337
CAGTCAC34150.031.01362427
CGCTATG38550.026.3101429
GTCTGAA45400.025.91020617
CTTAAAC35950.025.5415081
GTCGCTA40250.025.37059827
CTATAAG47600.025.00577246
ACCTATA36900.024.5890666
TATAAGC50550.023.54665247
AACCTAT41350.022.6937355
Produced by FastQC (version 0.11.2)