FastQCFastQC Report
Tue 14 Mar 2017
HYMN3BGXY_n01_4s-35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYMN3BGXY_n01_4s-35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16340278
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC176530.10803365768930002No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT35450.044.18276242
TCGTATG38950.042.25091643
ACGTCTG43200.042.16197215
GTCACGT43050.039.3476629
AGTCACG44250.038.12479828
TATGCCG44850.036.4620546
TCACGTA47400.035.80854830
CGTATGC47150.034.75690544
CACGTCT53400.034.56065414
ACGTAGA51050.033.18000432
ACACGTC57000.032.92244713
CGTAGAG52500.032.46023633
CAGTCAC53250.032.06989327
CACGTAG54050.031.40229831
GTATGCC54700.030.59001245
CACACGT68700.027.36578212
ATGCCGT61650.026.58212747
GTCTGAA72100.026.02729617
ATCGGAA70750.025.9922122
TGTTACC40950.025.60871313
Produced by FastQC (version 0.11.2)