FastQCFastQC Report
Tue 14 Mar 2017
HYMN3BGXY_n01_4s-34.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYMN3BGXY_n01_4s-34.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14208353
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC167160.11764910401648947No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT25500.041.53505744
TCGTATG29000.040.80407745
ACGTCTG33150.040.16803715
GTCACGT30200.038.72496429
TGAAACG30900.037.84676735
CACGTGA31150.037.76424831
TATGCCG32000.036.65797448
TCACGTG33150.036.0066930
AGTCACG34100.034.3975428
CACGTCT39100.034.23184614
GAAACGA35700.033.14453536
ACACGTC41900.032.6852513
CAGTCAC41400.028.74882327
TGTTACC36350.028.5652113
CGTATGC41600.028.3628246
GTATGCC42750.027.84204347
CGCTATG35000.026.9087129
TTACCTC38650.026.59767215
GTCTGAA51800.026.17188817
ATGCCGT46550.025.71917249
Produced by FastQC (version 0.11.2)