FastQCFastQC Report
Tue 14 Mar 2017
HYMN3BGXY_n01_4s-32.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYMN3BGXY_n01_4s-32.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14724448
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG201160.1366163268055957No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG154980.10525352121858829No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT25500.041.6702944
ACGTCTG30650.039.8431315
GTCACGT28000.039.42722329
TCGTATG28050.038.62017445
ACGTCCG30550.036.58645232
TCACGTC31200.035.824630
TATGCCG31650.034.8835248
AGTCACG32950.033.81838228
CGCACAT33300.033.35774237
CACGTCT37400.033.1133814
ACACGTC37800.032.3066613
CCGCACA35000.032.23032836
CGTATGC37850.028.9860546
CAGTCAC40550.027.82025527
CTATAAG59500.027.13667946
GTATGCC41550.026.65396547
CGCTATG41250.026.17728429
TATAAGC62900.025.8343847
GTCGCTA42200.025.50623327
TTTTTCC72450.025.1076771
Produced by FastQC (version 0.11.2)