FastQCFastQC Report
Tue 14 Mar 2017
HYMN3BGXY_n01_4s-25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYMN3BGXY_n01_4s-25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14171178
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC173790.1226362409674058No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGT26700.041.21719429
ACGTCTG29500.039.9930715
CTCGTAT24800.037.2827744
AGTCACG30550.036.47458328
TCACGTG31050.035.997730
TCGTATG27100.035.13651745
CACGTCT36200.032.78162414
TATCTCG29950.032.13741741
ACACGTC38050.031.27832813
TATGCCG30650.031.18053248
GCCTTAT34850.030.7853737
CAGTCAC38600.028.86773327
CGTATGC34800.026.96607846
TGTTACC37050.026.72223513
TTACCTC36100.026.66107215
CCTTATC39650.026.36266138
GTATGCC38950.024.80162847
GTCTGAA51000.024.2151617
CTTATCT42500.023.7832739
GTCGCTA39100.023.7341127
Produced by FastQC (version 0.11.2)