FastQCFastQC Report
Tue 14 Mar 2017
HYMN3BGXY_n01_4s-22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYMN3BGXY_n01_4s-22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14814566
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAATC25050.043.79360238
TACGTAA28250.041.88538736
ACGTCTG32650.039.72712315
ACGTAAT30050.039.03204337
TCGTATG28200.038.65931745
CTCGTAT27200.037.92514444
TATGCCG29650.036.65459448
CCGTACG34050.035.05407733
CACGTCT37650.034.6344114
ACACGTC39500.033.3615213
CAGTCAC40550.029.94730627
GTATGCC40100.028.82247747
CGTATGC38800.028.63192746
ACCGTAC45850.027.3115832
GTCTGAA50500.026.36771817
TAATCTC40850.026.34846340
ATGCCGT43800.024.49839649
CACACGT54350.024.30965212
CGCTATG44000.023.52209329
GTCGCTA44500.023.41277327
Produced by FastQC (version 0.11.2)