FastQCFastQC Report
Wed 22 May 2019
HYMM5BCX2_l02.1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYMM5BCX2_l02.1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112001043
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGA47738164.262296021654013Illumina Paired End PCR Primer 2 (100% over 32bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGATC5399000.041.71021318
CCGAGAT5473250.041.18667217
TGCCGAG5549200.040.6777515
GCCGAGA5586600.040.42321816
AGCGGTT5683900.039.34802630
GCGGTTC5680650.039.314531
GAGCGGT5755750.038.88581529
GAGATCG5845050.038.50718719
AGAGCGG5824850.038.47936628
AAGAGCG5876900.038.13623427
CGGAAGA6019800.037.30061324
TCGGAAG6033050.037.21869723
ATCGGAA6094250.036.87724322
GATCGGA6150800.036.56488421
GAAGAGC6141600.036.56094426
AGATCGG6223300.036.16638620
GGAAGAG6426350.035.0094925
GAGACCG179150.022.85001645
ATGCCGA10364900.021.7633114
AATGCCG10453950.021.59001413
Produced by FastQC (version 0.11.5)