FastQCFastQC Report
Wed 22 May 2019
HYMM5BCX2_l01.1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYMM5BCX2_l01.1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129250922
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGA2797409321.643244448190472Illumina Paired End PCR Primer 2 (100% over 32bp)
CAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCG8520490.6592208293879714Illumina Paired End PCR Primer 2 (100% over 37bp)
CGTTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGA2528510.19562800488185297Illumina Paired End PCR Primer 2 (100% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGAT32333700.042.22327417
CGAGATC32551700.041.87764718
GCCGAGA33511650.040.8237216
TGCCGAG33612800.040.6674315
GCGGTTC35161800.038.40522831
GAGATCG35571650.038.31858419
AGCGGTT35436550.038.17960430
GAGCGGT35783000.037.84465829
AGAGCGG36177450.037.4861628
AAGAGCG36508200.037.1876627
GAAGAGC37090700.036.6502126
CGGAAGA37646200.036.13978624
GGAAGAG37729250.036.10243225
TCGGAAG37853750.035.95097723
ATCGGAA38255900.035.6084322
GAGACCG1175650.035.3635645
GATCGGA38695800.035.23077821
AGATCGG39125450.034.88339220
CGTTTCA351650.034.541871
CCGAGAC1271150.032.77067643
Produced by FastQC (version 0.11.5)