FastQCFastQC Report
Thu 9 May 2024
HYLWKDRX3_n02_EV-exp-1-NEB_a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYLWKDRX3_n02_EV-exp-1-NEB_a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1941795
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC134340.6918341019520599Illumina Single End PCR Primer 1 (100% over 50bp)
CTATCCCCTGAGACTGCCAAGGCACACAGGGGATAGAGATCGGAAGAGCG58230.29987717549998844No Hit
TCGCACATTCCGCTTCTACCGGGGGGGTCGAGCTGAGTGGATGCGAATCT52590.2708318849312105No Hit
CGCACATTCCGCTTCTACCGGGGGGGTCGAGCTGAGTGGATGCGAATCTG45710.23540075033667301No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37530.1932747792635165No Hit
ATCCCCTGAGACTGCCAAGGCACACAGGGGATAGATCGGAAGAGCGTCGT32330.16649543334904046No Hit
CACGGACTTACGGCCCACCCACAGATTCGCATCCACTCAGCTCGACCCCC32130.165465458506176No Hit
GCACATTCCGCTTCTACCGGGGGGGTCGAGCTGAGTGGATGCGAATCTGT22860.11772612453940813No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)