FastQCFastQC Report
Thu 9 May 2024
HYLWKDRX3_n02_EV-3-h-NEB_a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYLWKDRX3_n02_EV-3-h-NEB_a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12823379
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG157720.12299410319230213No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC147060.11468116165013918Illumina Single End PCR Primer 1 (100% over 50bp)
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA132360.10321772443908894No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)