FastQCFastQC Report
Thu 9 May 2024
HYLWKDRX3_n01_EV-exp-1-NEB_a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYLWKDRX3_n01_EV-exp-1-NEB_a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1941795
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAAGGTGATCTCGTAT262491.3517904825174645TruSeq Adapter, Index 19 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAAGGTGATCTCGTA98430.5069021189157454TruSeq Adapter, Index 19 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAAGGTGATCGCGTAT82310.4238861465808698TruSeq Adapter, Index 19 (97% over 37bp)
CTATCCCCTGTGTGCCTTGGCAGTCTCAGGGGATAGAGATCGGAAGAGCA47600.2451340126017422No Hit
CGCACATTCCGCTTCTACCGGGGGGGTCGAGCTGAGTGGATGCGAATCTG43610.22458601448659618No Hit
CACGGACTTACGGCCCACCCACAGATTCGCATCCACTCAGCTCGACCCCC39680.20434700882430945No Hit
TCGCACATTCCGCTTCTACCGGGGGGGTCGAGCTGAGTGGATGCGAATCT33620.17313877108551623No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAAGGTGATCGCGTA32710.16845238555048295TruSeq Adapter, Index 19 (97% over 37bp)
ATCCCCTGTGTGCCTTGGCAGTCTCAGGGGATAGATCGGAAGAGCACACG31030.15980059687042145No Hit
GCACATTCCGCTTCTACCGGGGGGGTCGAGCTGAGTGGATGCGAATCTGT21890.11273074655151548No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)