FastQCFastQC Report
Thu 9 May 2024
HYLWKDRX3_n01_EV-1-h-NEB_a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYLWKDRX3_n01_EV-1-h-NEB_a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19343094
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAATTGCATCTCGTAT285010.14734457682933247TruSeq Adapter, Index 22 (97% over 37bp)
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG256570.13264165494930646No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA245290.12681011631334677No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC200720.1037683009760486No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTC193750.10016494775861608No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)