FastQCFastQC Report
Thu 9 May 2024
HYLWKDRX3_n01_8G_1-6-NEB_a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYLWKDRX3_n01_8G_1-6-NEB_a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6996734
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAACCGAATCTCGGTT1069341.5283416519764792TruSeq Adapter, Index 19 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAACCGAATCTCGGGT880851.258944530405186TruSeq Adapter, Index 19 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAACCGAATCTCGGT591460.8453372673593137TruSeq Adapter, Index 19 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAACCGAATCTCGGG453130.6476307374269195TruSeq Adapter, Index 19 (97% over 37bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT442210.6320234555151018No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAACCGAATCGCGGTT396390.5665357579693611TruSeq Adapter, Index 19 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAACCGAATCGCGGGT295240.42196830692720344TruSeq Adapter, Index 19 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAACCGAATCGCGGG200050.28591911597611114TruSeq Adapter, Index 19 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAACCGAATCGCGGT199990.2858333616798924TruSeq Adapter, Index 19 (97% over 37bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80830.11552532938939797No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)