FastQCFastQC Report
Thu 9 May 2024
HYLWKDRX3_n01_7R_1-6-NEB_a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYLWKDRX3_n01_7R_1-6-NEB_a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8870476
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATCTCCATCTCGTAT3358703.7863807985050633TruSeq Adapter, Index 3 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATCTCCATCGCGTAT1501951.693201131483812TruSeq Adapter, Index 3 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATCTCCATCTCGTA535900.6041389436147508TruSeq Adapter, Index 3 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATCTCCATCGCGTA241130.2718343412461744TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)