FastQCFastQC Report
Thu 9 May 2024
HYLWKDRX3_n01_1R_1-6-NEB_a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYLWKDRX3_n01_1R_1-6-NEB_a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11834591
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG277030.23408498020759655No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC213240.18018366667677826No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA191180.161543394275307No Hit
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCT156210.13199442211395393No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT155440.13134378703919722No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG149210.1260795577979839No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC146740.12399245567506305No Hit
CAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTC136210.115094809782611No Hit
CAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCG127610.10782797648013352No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCT125970.10644220826896342No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG119020.10056959298382175No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)