FastQCFastQC Report
Thu 9 May 2024
HYLWKDRX3_n01_15R_1-6-NEB_a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYLWKDRX3_n01_15R_1-6-NEB_a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16790075
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTAAGCATCTCGTAT6034323.5939803723330597TruSeq Adapter, Index 11 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTAAGCATCGCGTAT2286201.3616377532560158TruSeq Adapter, Index 11 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTAAGCATCTCGTA923640.550110705282734TruSeq Adapter, Index 11 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTAAGCATCGCGTA374190.22286380495620178TruSeq Adapter, Index 11 (97% over 38bp)
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG191670.1141567265184938No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)