FastQCFastQC Report
Thu 9 May 2024
HYLWKDRX3_n01_14R_1-7-NEB_a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYLWKDRX3_n01_14R_1-7-NEB_a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5709484
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACATCAATCTCGTAT1725173.0215865391688634TruSeq Adapter, Index 4 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACATCAATCGCGTAT539460.9448489565782127TruSeq Adapter, Index 4 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACATCAATCTCGTTT222060.38893181940784843TruSeq Adapter, Index 4 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACATCAATCTCGGAT177280.3105009139179653TruSeq Adapter, Index 4 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACATCAATCTCGTA109450.19169858432040443TruSeq Adapter, Index 4 (97% over 37bp)
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG73660.12901340996839644No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACATCAATCGCGTTT66110.11578979816740008TruSeq Adapter, Index 4 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)