FastQCFastQC Report
Thu 9 May 2024
HYLWKDRX3_n01_12R_1-7-NEB_a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYLWKDRX3_n01_12R_1-7-NEB_a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19735505
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTCCAATCTCGTAT2622311.3287270834974834TruSeq Adapter, Index 23 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTCCAATCTCGTTT1974351.0004051074446791TruSeq Adapter, Index 23 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTCCAATCTCGGTT1195410.6057154352016835TruSeq Adapter, Index 23 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTCCAATCTCGGAT1174950.5953483328650572TruSeq Adapter, Index 23 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTCCAATCGCGTAT848900.4301384737811371TruSeq Adapter, Index 23 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTCCAATCGCGTTT616110.31218354939485965TruSeq Adapter, Index 23 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTCCAATCTCGTT468500.23738941567494726TruSeq Adapter, Index 23 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTCCAATCTCGTA366700.18580725448880075TruSeq Adapter, Index 23 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTCCAATCGCGGAT286140.14498742241457718TruSeq Adapter, Index 23 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTCCAATCGCGGTT269060.13633296943756948TruSeq Adapter, Index 23 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTCCAATCTCGGT260500.13199560892918624TruSeq Adapter, Index 23 (97% over 39bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)