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        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

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        About MultiQC

        This report was generated using MultiQC, version 1.9

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        MultiQC is published in Bioinformatics:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

        A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.

        Report generated on 2024-04-24, 16:32 based on data in: /vast/gencore/GENEFLOW/work/72/9bb39c5166a62cba79db7f70ce3337/merged


        General Statistics

        Showing 272/272 rows and 3/5 columns.
        Sample Name% Dups% GCM Seqs
        HYL55DRX3_n01_AtTGA5_Col-B_aiqiagen
        19.5%
        36%
        0.6
        HYL55DRX3_n01_AtbZIP12_An1AmpPoolHm-B_ai1
        56.7%
        38%
        5.8
        HYL55DRX3_n01_AtbZIP12_An1AmpPoolHm-B_ai2
        50.1%
        38%
        3.6
        HYL55DRX3_n01_AtbZIP12_C24AmpPoolHm-B_ai1
        51.3%
        38%
        2.2
        HYL55DRX3_n01_AtbZIP12_C24AmpPoolHm-B_ai2
        43.6%
        38%
        1.3
        HYL55DRX3_n01_AtbZIP12_ColAmpPoolHm-B_ai1
        56.4%
        38%
        9.0
        HYL55DRX3_n01_AtbZIP12_ColAmpPoolHm-B_ai2
        50.1%
        38%
        5.7
        HYL55DRX3_n01_AtbZIP12_CviAmpPoolHm-B_ai1
        56.7%
        38%
        5.9
        HYL55DRX3_n01_AtbZIP12_CviAmpPoolHm-B_ai2
        49.7%
        38%
        3.5
        HYL55DRX3_n01_AtbZIP12_EriAmpPoolHm-B_ai1
        51.3%
        37%
        3.4
        HYL55DRX3_n01_AtbZIP12_EriAmpPoolHm-B_ai2
        43.7%
        38%
        2.0
        HYL55DRX3_n01_AtbZIP12_KyoAmpPoolHm-B_ai1
        53.9%
        38%
        4.8
        HYL55DRX3_n01_AtbZIP12_KyoAmpPoolHm-B_ai2
        47.2%
        39%
        3.0
        HYL55DRX3_n01_AtbZIP12_LerAmpPoolHm-B_ai1
        53.3%
        38%
        4.0
        HYL55DRX3_n01_AtbZIP12_LerAmpPoolHm-B_ai2
        46.4%
        38%
        2.5
        HYL55DRX3_n01_AtbZIP12_ShaAmpPoolHm-B_ai1
        54.2%
        38%
        3.6
        HYL55DRX3_n01_AtbZIP12_ShaAmpPoolHm-B_ai2
        46.6%
        38%
        2.2
        HYL55DRX3_n01_AtbZIP20_An1AmpPoolHm-B_ai1
        44.5%
        38%
        4.4
        HYL55DRX3_n01_AtbZIP20_An1AmpPoolHm-B_ai2
        42.6%
        38%
        4.5
        HYL55DRX3_n01_AtbZIP20_C24AmpPoolHm-B_ai1
        41.9%
        37%
        2.3
        HYL55DRX3_n01_AtbZIP20_C24AmpPoolHm-B_ai2
        39.9%
        37%
        2.5
        HYL55DRX3_n01_AtbZIP20_ColAmpPoolHm-B_ai1
        45.9%
        38%
        7.8
        HYL55DRX3_n01_AtbZIP20_ColAmpPoolHm-B_ai2
        44.1%
        38%
        7.7
        HYL55DRX3_n01_AtbZIP20_CviAmpPoolHm-B_ai1
        44.2%
        37%
        3.9
        HYL55DRX3_n01_AtbZIP20_CviAmpPoolHm-B_ai2
        42.2%
        38%
        4.0
        HYL55DRX3_n01_AtbZIP20_EriAmpPoolHm-B_ai1
        42.1%
        37%
        2.9
        HYL55DRX3_n01_AtbZIP20_EriAmpPoolHm-B_ai2
        39.8%
        37%
        3.0
        HYL55DRX3_n01_AtbZIP20_KyoAmpPoolHm-B_ai1
        44.3%
        38%
        5.0
        HYL55DRX3_n01_AtbZIP20_KyoAmpPoolHm-B_ai2
        42.3%
        38%
        5.0
        HYL55DRX3_n01_AtbZIP20_LerAmpPoolHm-B_ai1
        44.8%
        38%
        3.7
        HYL55DRX3_n01_AtbZIP20_LerAmpPoolHm-B_ai2
        42.7%
        38%
        3.8
        HYL55DRX3_n01_AtbZIP20_ShaAmpPoolHm-B_ai1
        42.8%
        37%
        2.6
        HYL55DRX3_n01_AtbZIP20_ShaAmpPoolHm-B_ai2
        40.5%
        38%
        2.8
        HYL55DRX3_n01_AtbZIP26_An1AmpPoolHm-B_ai
        28.8%
        39%
        6.0
        HYL55DRX3_n01_AtbZIP26_C24AmpPoolHm-B_ai
        30.2%
        39%
        9.0
        HYL55DRX3_n01_AtbZIP26_ColAmpPoolHm-B_ai
        28.3%
        40%
        6.1
        HYL55DRX3_n01_AtbZIP26_CviAmpPoolHm-B_ai
        29.5%
        39%
        6.9
        HYL55DRX3_n01_AtbZIP26_EriAmpPoolHm-B_ai
        23.5%
        38%
        5.7
        HYL55DRX3_n01_AtbZIP26_KyoAmpPoolHm-B_ai
        29.8%
        40%
        6.5
        HYL55DRX3_n01_AtbZIP26_LerAmpPoolHm-B_ai
        31.2%
        39%
        8.2
        HYL55DRX3_n01_AtbZIP26_ShaAmpPoolHm-B_ai
        24.9%
        39%
        7.0
        HYL55DRX3_n01_AtbZIP45_An1AmpPoolHm-B_ai1
        48.3%
        38%
        5.9
        HYL55DRX3_n01_AtbZIP45_An1AmpPoolHm-B_ai2
        45.6%
        38%
        4.0
        HYL55DRX3_n01_AtbZIP45_C24AmpPoolHm-B_ai1
        48.7%
        37%
        3.5
        HYL55DRX3_n01_AtbZIP45_C24AmpPoolHm-B_ai2
        46.7%
        38%
        2.4
        HYL55DRX3_n01_AtbZIP45_ColAmpPoolHm-B_ai1
        48.7%
        38%
        10.1
        HYL55DRX3_n01_AtbZIP45_ColAmpPoolHm-B_ai2
        46.0%
        38%
        6.7
        HYL55DRX3_n01_AtbZIP45_CviAmpPoolHm-B_ai1
        48.1%
        38%
        5.5
        HYL55DRX3_n01_AtbZIP45_CviAmpPoolHm-B_ai2
        45.6%
        38%
        3.7
        HYL55DRX3_n01_AtbZIP45_EriAmpPoolHm-B_ai1
        48.0%
        37%
        4.4
        HYL55DRX3_n01_AtbZIP45_EriAmpPoolHm-B_ai2
        45.5%
        37%
        2.9
        HYL55DRX3_n01_AtbZIP45_KyoAmpPoolHm-B_ai1
        47.4%
        38%
        6.5
        HYL55DRX3_n01_AtbZIP45_KyoAmpPoolHm-B_ai2
        44.9%
        38%
        4.3
        HYL55DRX3_n01_AtbZIP45_LerAmpPoolHm-B_ai1
        49.1%
        38%
        5.2
        HYL55DRX3_n01_AtbZIP45_LerAmpPoolHm-B_ai2
        46.3%
        38%
        3.4
        HYL55DRX3_n01_AtbZIP45_ShaAmpPoolHm-B_ai1
        49.1%
        38%
        4.0
        HYL55DRX3_n01_AtbZIP45_ShaAmpPoolHm-B_ai2
        46.5%
        38%
        2.7
        HYL55DRX3_n01_AtbZIP53_An1AmpPoolHm-B_ai1
        69.7%
        38%
        3.7
        HYL55DRX3_n01_AtbZIP53_An1AmpPoolHm-B_ai2
        31.2%
        38%
        4.1
        HYL55DRX3_n01_AtbZIP53_C24AmpPoolHm-B_ai1
        71.5%
        37%
        5.0
        HYL55DRX3_n01_AtbZIP53_C24AmpPoolHm-B_ai2
        30.6%
        38%
        4.2
        HYL55DRX3_n01_AtbZIP53_ColAmpPoolHm-B_ai1
        69.5%
        38%
        3.9
        HYL55DRX3_n01_AtbZIP53_ColAmpPoolHm-B_ai2
        30.8%
        38%
        4.5
        HYL55DRX3_n01_AtbZIP53_CviAmpPoolHm-B_ai1
        70.4%
        37%
        4.3
        HYL55DRX3_n01_AtbZIP53_CviAmpPoolHm-B_ai2
        31.9%
        38%
        4.7
        HYL55DRX3_n01_AtbZIP53_EriAmpPoolHm-B_ai1
        69.0%
        37%
        3.6
        HYL55DRX3_n01_AtbZIP53_EriAmpPoolHm-B_ai2
        26.8%
        37%
        3.8
        HYL55DRX3_n01_AtbZIP53_KyoAmpPoolHm-B_ai1
        70.1%
        38%
        4.0
        HYL55DRX3_n01_AtbZIP53_KyoAmpPoolHm-B_ai2
        31.5%
        39%
        4.4
        HYL55DRX3_n01_AtbZIP53_LerAmpPoolHm-B_ai1
        70.8%
        38%
        5.1
        HYL55DRX3_n01_AtbZIP53_LerAmpPoolHm-B_ai2
        33.4%
        38%
        5.2
        HYL55DRX3_n01_AtbZIP53_ShaAmpPoolHm-B_ai1
        69.3%
        37%
        4.1
        HYL55DRX3_n01_AtbZIP53_ShaAmpPoolHm-B_ai2
        28.3%
        38%
        4.2
        HYL55DRX3_n01_AtbZIP56_An1AmpPoolHm-B_ai1
        38.8%
        42%
        11.7
        HYL55DRX3_n01_AtbZIP56_An1AmpPoolHm-B_ai2
        39.5%
        43%
        9.3
        HYL55DRX3_n01_AtbZIP56_C24AmpPoolHm-B_ai1
        39.3%
        42%
        14.1
        HYL55DRX3_n01_AtbZIP56_C24AmpPoolHm-B_ai2
        39.4%
        42%
        11.3
        HYL55DRX3_n01_AtbZIP56_ColAmpPoolHm-B_ai1
        37.5%
        42%
        15.4
        HYL55DRX3_n01_AtbZIP56_ColAmpPoolHm-B_ai2
        37.3%
        42%
        12.3
        HYL55DRX3_n01_AtbZIP56_CviAmpPoolHm-B_ai1
        37.3%
        41%
        12.6
        HYL55DRX3_n01_AtbZIP56_CviAmpPoolHm-B_ai2
        37.1%
        42%
        10.1
        HYL55DRX3_n01_AtbZIP56_EriAmpPoolHm-B_ai1
        30.4%
        40%
        10.5
        HYL55DRX3_n01_AtbZIP56_EriAmpPoolHm-B_ai2
        30.1%
        41%
        8.1
        HYL55DRX3_n01_AtbZIP56_KyoAmpPoolHm-B_ai1
        39.6%
        42%
        11.5
        HYL55DRX3_n01_AtbZIP56_KyoAmpPoolHm-B_ai2
        40.0%
        43%
        9.2
        HYL55DRX3_n01_AtbZIP56_LerAmpPoolHm-B_ai1
        37.1%
        42%
        12.0
        HYL55DRX3_n01_AtbZIP56_LerAmpPoolHm-B_ai2
        36.8%
        43%
        9.5
        HYL55DRX3_n01_AtbZIP56_ShaAmpPoolHm-B_ai1
        36.0%
        41%
        15.9
        HYL55DRX3_n01_AtbZIP56_ShaAmpPoolHm-B_ai2
        36.2%
        42%
        12.6
        HYL55DRX3_n01_AtbZIP57_An1AmpPoolHm-B_ai1
        51.2%
        37%
        5.5
        HYL55DRX3_n01_AtbZIP57_An1AmpPoolHm-B_ai2
        53.2%
        38%
        5.9
        HYL55DRX3_n01_AtbZIP57_C24AmpPoolHm-B_ai1
        51.0%
        37%
        3.1
        HYL55DRX3_n01_AtbZIP57_C24AmpPoolHm-B_ai2
        54.0%
        38%
        3.4
        HYL55DRX3_n01_AtbZIP57_ColAmpPoolHm-B_ai1
        51.4%
        37%
        9.5
        HYL55DRX3_n01_AtbZIP57_ColAmpPoolHm-B_ai2
        52.9%
        38%
        9.8
        HYL55DRX3_n01_AtbZIP57_CviAmpPoolHm-B_ai1
        51.0%
        37%
        5.0
        HYL55DRX3_n01_AtbZIP57_CviAmpPoolHm-B_ai2
        53.0%
        38%
        5.3
        HYL55DRX3_n01_AtbZIP57_EriAmpPoolHm-B_ai1
        51.0%
        36%
        3.9
        HYL55DRX3_n01_AtbZIP57_EriAmpPoolHm-B_ai2
        53.5%
        38%
        4.2
        HYL55DRX3_n01_AtbZIP57_KyoAmpPoolHm-B_ai1
        50.2%
        37%
        6.1
        HYL55DRX3_n01_AtbZIP57_KyoAmpPoolHm-B_ai2
        52.0%
        38%
        6.4
        HYL55DRX3_n01_AtbZIP57_LerAmpPoolHm-B_ai1
        51.6%
        37%
        4.7
        HYL55DRX3_n01_AtbZIP57_LerAmpPoolHm-B_ai2
        54.0%
        38%
        5.0
        HYL55DRX3_n01_AtbZIP57_ShaAmpPoolHm-B_ai1
        51.8%
        37%
        3.5
        HYL55DRX3_n01_AtbZIP57_ShaAmpPoolHm-B_ai2
        55.0%
        38%
        3.9
        HYL55DRX3_n01_AtbZIP68_An1AmpPoolHm-B_ai1
        47.8%
        38%
        4.3
        HYL55DRX3_n01_AtbZIP68_An1AmpPoolHm-B_ai2
        38.8%
        38%
        2.0
        HYL55DRX3_n01_AtbZIP68_C24AmpPoolHm-B_ai1
        50.5%
        37%
        3.1
        HYL55DRX3_n01_AtbZIP68_C24AmpPoolHm-B_ai2
        40.0%
        37%
        1.3
        HYL55DRX3_n01_AtbZIP68_ColAmpPoolHm-B_ai1
        46.0%
        37%
        6.6
        HYL55DRX3_n01_AtbZIP68_ColAmpPoolHm-B_ai2
        38.1%
        38%
        3.4
        HYL55DRX3_n01_AtbZIP68_CviAmpPoolHm-B_ai1
        48.0%
        37%
        4.0
        HYL55DRX3_n01_AtbZIP68_CviAmpPoolHm-B_ai2
        38.5%
        37%
        1.9
        HYL55DRX3_n01_AtbZIP68_EriAmpPoolHm-B_ai1
        49.2%
        37%
        3.5
        HYL55DRX3_n01_AtbZIP68_EriAmpPoolHm-B_ai2
        38.5%
        37%
        1.5
        HYL55DRX3_n01_AtbZIP68_KyoAmpPoolHm-B_ai1
        46.0%
        38%
        4.4
        HYL55DRX3_n01_AtbZIP68_KyoAmpPoolHm-B_ai2
        37.3%
        38%
        2.2
        HYL55DRX3_n01_AtbZIP68_LerAmpPoolHm-B_ai1
        48.6%
        37%
        3.9
        HYL55DRX3_n01_AtbZIP68_LerAmpPoolHm-B_ai2
        38.7%
        38%
        1.8
        HYL55DRX3_n01_AtbZIP68_ShaAmpPoolHm-B_ai1
        50.4%
        37%
        3.3
        HYL55DRX3_n01_AtbZIP68_ShaAmpPoolHm-B_ai2
        40.4%
        38%
        1.4
        HYL55DRX3_n01_PdaTF1_Pda-B_ai1
        65.1%
        38%
        42.7
        HYL55DRX3_n01_PdaTF1_Pda-B_ai2
        70.2%
        37%
        14.1
        HYL55DRX3_n01_PdaTF2_Pda-B_ai1
        63.2%
        38%
        32.7
        HYL55DRX3_n01_PdaTF2_Pda-B_ai2
        60.7%
        37%
        23.7
        HYL55DRX3_n01_pIXHALO_An1AmpPoolHm-B_ai
        27.1%
        38%
        3.3
        HYL55DRX3_n01_pIXHALO_C24AmpPoolHm-B_ai
        26.6%
        37%
        3.6
        HYL55DRX3_n01_pIXHALO_ColAmpPoolHm-B_ai
        26.9%
        38%
        3.5
        HYL55DRX3_n01_pIXHALO_CviAmpPoolHm-B_ai
        28.0%
        37%
        3.7
        HYL55DRX3_n01_pIXHALO_EriAmpPoolHm-B_ai
        22.4%
        36%
        3.0
        HYL55DRX3_n01_pIXHALO_KyoAmpPoolHm-B_ai
        27.9%
        38%
        3.6
        HYL55DRX3_n01_pIXHALO_LerAmpPoolHm-B_ai
        30.1%
        37%
        4.3
        HYL55DRX3_n01_pIXHALO_Pda-B_ai1
        31.5%
        42%
        5.2
        HYL55DRX3_n01_pIXHALO_Pda-B_ai2
        28.0%
        42%
        5.5
        HYL55DRX3_n01_pIXHALO_ShaAmpPoolHm-B_ai
        23.2%
        37%
        3.4
        HYL55DRX3_n01_undetermined
        73.9%
        43%
        96.8
        HYL55DRX3_n02_AtTGA5_Col-B_aiqiagen
        19.4%
        36%
        0.6
        HYL55DRX3_n02_AtbZIP12_An1AmpPoolHm-B_ai1
        54.6%
        38%
        5.8
        HYL55DRX3_n02_AtbZIP12_An1AmpPoolHm-B_ai2
        48.0%
        38%
        3.6
        HYL55DRX3_n02_AtbZIP12_C24AmpPoolHm-B_ai1
        49.7%
        38%
        2.2
        HYL55DRX3_n02_AtbZIP12_C24AmpPoolHm-B_ai2
        42.0%
        38%
        1.3
        HYL55DRX3_n02_AtbZIP12_ColAmpPoolHm-B_ai1
        54.7%
        38%
        9.0
        HYL55DRX3_n02_AtbZIP12_ColAmpPoolHm-B_ai2
        48.5%
        38%
        5.7
        HYL55DRX3_n02_AtbZIP12_CviAmpPoolHm-B_ai1
        54.8%
        38%
        5.9
        HYL55DRX3_n02_AtbZIP12_CviAmpPoolHm-B_ai2
        47.8%
        38%
        3.5
        HYL55DRX3_n02_AtbZIP12_EriAmpPoolHm-B_ai1
        49.5%
        37%
        3.4
        HYL55DRX3_n02_AtbZIP12_EriAmpPoolHm-B_ai2
        41.9%
        38%
        2.0
        HYL55DRX3_n02_AtbZIP12_KyoAmpPoolHm-B_ai1
        51.9%
        38%
        4.8
        HYL55DRX3_n02_AtbZIP12_KyoAmpPoolHm-B_ai2
        45.2%
        39%
        3.0
        HYL55DRX3_n02_AtbZIP12_LerAmpPoolHm-B_ai1
        51.3%
        38%
        4.0
        HYL55DRX3_n02_AtbZIP12_LerAmpPoolHm-B_ai2
        44.5%
        38%
        2.5
        HYL55DRX3_n02_AtbZIP12_ShaAmpPoolHm-B_ai1
        52.1%
        38%
        3.6
        HYL55DRX3_n02_AtbZIP12_ShaAmpPoolHm-B_ai2
        44.7%
        38%
        2.2
        HYL55DRX3_n02_AtbZIP20_An1AmpPoolHm-B_ai1
        42.8%
        38%
        4.4
        HYL55DRX3_n02_AtbZIP20_An1AmpPoolHm-B_ai2
        41.0%
        38%
        4.5
        HYL55DRX3_n02_AtbZIP20_C24AmpPoolHm-B_ai1
        40.5%
        37%
        2.3
        HYL55DRX3_n02_AtbZIP20_C24AmpPoolHm-B_ai2
        38.9%
        37%
        2.5
        HYL55DRX3_n02_AtbZIP20_ColAmpPoolHm-B_ai1
        44.6%
        38%
        7.8
        HYL55DRX3_n02_AtbZIP20_ColAmpPoolHm-B_ai2
        42.7%
        38%
        7.7
        HYL55DRX3_n02_AtbZIP20_CviAmpPoolHm-B_ai1
        42.5%
        38%
        3.9
        HYL55DRX3_n02_AtbZIP20_CviAmpPoolHm-B_ai2
        40.8%
        38%
        4.0
        HYL55DRX3_n02_AtbZIP20_EriAmpPoolHm-B_ai1
        40.8%
        37%
        2.9
        HYL55DRX3_n02_AtbZIP20_EriAmpPoolHm-B_ai2
        38.5%
        37%
        3.0
        HYL55DRX3_n02_AtbZIP20_KyoAmpPoolHm-B_ai1
        42.6%
        38%
        5.0
        HYL55DRX3_n02_AtbZIP20_KyoAmpPoolHm-B_ai2
        40.9%
        38%
        5.0
        HYL55DRX3_n02_AtbZIP20_LerAmpPoolHm-B_ai1
        43.0%
        38%
        3.7
        HYL55DRX3_n02_AtbZIP20_LerAmpPoolHm-B_ai2
        41.3%
        38%
        3.8
        HYL55DRX3_n02_AtbZIP20_ShaAmpPoolHm-B_ai1
        41.1%
        37%
        2.6
        HYL55DRX3_n02_AtbZIP20_ShaAmpPoolHm-B_ai2
        39.1%
        37%
        2.8
        HYL55DRX3_n02_AtbZIP26_An1AmpPoolHm-B_ai
        28.3%
        39%
        6.0
        HYL55DRX3_n02_AtbZIP26_C24AmpPoolHm-B_ai
        30.0%
        39%
        9.0
        HYL55DRX3_n02_AtbZIP26_ColAmpPoolHm-B_ai
        27.8%
        39%
        6.1
        HYL55DRX3_n02_AtbZIP26_CviAmpPoolHm-B_ai
        29.1%
        39%
        6.9
        HYL55DRX3_n02_AtbZIP26_EriAmpPoolHm-B_ai
        23.1%
        38%
        5.7
        HYL55DRX3_n02_AtbZIP26_KyoAmpPoolHm-B_ai
        29.2%
        40%
        6.5
        HYL55DRX3_n02_AtbZIP26_LerAmpPoolHm-B_ai
        30.7%
        39%
        8.2
        HYL55DRX3_n02_AtbZIP26_ShaAmpPoolHm-B_ai
        24.5%
        39%
        7.0
        HYL55DRX3_n02_AtbZIP45_An1AmpPoolHm-B_ai1
        46.5%
        38%
        5.9
        HYL55DRX3_n02_AtbZIP45_An1AmpPoolHm-B_ai2
        44.0%
        38%
        4.0
        HYL55DRX3_n02_AtbZIP45_C24AmpPoolHm-B_ai1
        47.5%
        37%
        3.5
        HYL55DRX3_n02_AtbZIP45_C24AmpPoolHm-B_ai2
        45.3%
        37%
        2.4
        HYL55DRX3_n02_AtbZIP45_ColAmpPoolHm-B_ai1
        47.2%
        38%
        10.1
        HYL55DRX3_n02_AtbZIP45_ColAmpPoolHm-B_ai2
        44.6%
        38%
        6.7
        HYL55DRX3_n02_AtbZIP45_CviAmpPoolHm-B_ai1
        46.5%
        38%
        5.5
        HYL55DRX3_n02_AtbZIP45_CviAmpPoolHm-B_ai2
        44.0%
        38%
        3.7
        HYL55DRX3_n02_AtbZIP45_EriAmpPoolHm-B_ai1
        46.5%
        37%
        4.4
        HYL55DRX3_n02_AtbZIP45_EriAmpPoolHm-B_ai2
        43.9%
        37%
        2.9
        HYL55DRX3_n02_AtbZIP45_KyoAmpPoolHm-B_ai1
        45.7%
        38%
        6.5
        HYL55DRX3_n02_AtbZIP45_KyoAmpPoolHm-B_ai2
        43.2%
        38%
        4.3
        HYL55DRX3_n02_AtbZIP45_LerAmpPoolHm-B_ai1
        47.4%
        38%
        5.2
        HYL55DRX3_n02_AtbZIP45_LerAmpPoolHm-B_ai2
        44.5%
        38%
        3.4
        HYL55DRX3_n02_AtbZIP45_ShaAmpPoolHm-B_ai1
        47.5%
        38%
        4.0
        HYL55DRX3_n02_AtbZIP45_ShaAmpPoolHm-B_ai2
        45.0%
        38%
        2.7
        HYL55DRX3_n02_AtbZIP53_An1AmpPoolHm-B_ai1
        68.8%
        39%
        3.7
        HYL55DRX3_n02_AtbZIP53_An1AmpPoolHm-B_ai2
        30.1%
        38%
        4.1
        HYL55DRX3_n02_AtbZIP53_C24AmpPoolHm-B_ai1
        70.8%
        37%
        5.0
        HYL55DRX3_n02_AtbZIP53_C24AmpPoolHm-B_ai2
        29.5%
        37%
        4.2
        HYL55DRX3_n02_AtbZIP53_ColAmpPoolHm-B_ai1
        68.4%
        38%
        3.9
        HYL55DRX3_n02_AtbZIP53_ColAmpPoolHm-B_ai2
        29.7%
        38%
        4.5
        HYL55DRX3_n02_AtbZIP53_CviAmpPoolHm-B_ai1
        69.1%
        38%
        4.3
        HYL55DRX3_n02_AtbZIP53_CviAmpPoolHm-B_ai2
        30.7%
        38%
        4.7
        HYL55DRX3_n02_AtbZIP53_EriAmpPoolHm-B_ai1
        68.1%
        37%
        3.6
        HYL55DRX3_n02_AtbZIP53_EriAmpPoolHm-B_ai2
        25.9%
        37%
        3.8
        HYL55DRX3_n02_AtbZIP53_KyoAmpPoolHm-B_ai1
        68.8%
        38%
        4.0
        HYL55DRX3_n02_AtbZIP53_KyoAmpPoolHm-B_ai2
        30.3%
        38%
        4.4
        HYL55DRX3_n02_AtbZIP53_LerAmpPoolHm-B_ai1
        69.5%
        38%
        5.1
        HYL55DRX3_n02_AtbZIP53_LerAmpPoolHm-B_ai2
        32.2%
        38%
        5.2
        HYL55DRX3_n02_AtbZIP53_ShaAmpPoolHm-B_ai1
        68.0%
        37%
        4.1
        HYL55DRX3_n02_AtbZIP53_ShaAmpPoolHm-B_ai2
        27.0%
        38%
        4.2
        HYL55DRX3_n02_AtbZIP56_An1AmpPoolHm-B_ai1
        38.4%
        42%
        11.7
        HYL55DRX3_n02_AtbZIP56_An1AmpPoolHm-B_ai2
        38.7%
        43%
        9.3
        HYL55DRX3_n02_AtbZIP56_C24AmpPoolHm-B_ai1
        39.0%
        42%
        14.1
        HYL55DRX3_n02_AtbZIP56_C24AmpPoolHm-B_ai2
        38.8%
        42%
        11.3
        HYL55DRX3_n02_AtbZIP56_ColAmpPoolHm-B_ai1
        37.1%
        41%
        15.4
        HYL55DRX3_n02_AtbZIP56_ColAmpPoolHm-B_ai2
        36.7%
        42%
        12.3
        HYL55DRX3_n02_AtbZIP56_CviAmpPoolHm-B_ai1
        36.8%
        41%
        12.6
        HYL55DRX3_n02_AtbZIP56_CviAmpPoolHm-B_ai2
        36.6%
        42%
        10.1
        HYL55DRX3_n02_AtbZIP56_EriAmpPoolHm-B_ai1
        29.8%
        40%
        10.5
        HYL55DRX3_n02_AtbZIP56_EriAmpPoolHm-B_ai2
        29.3%
        41%
        8.1
        HYL55DRX3_n02_AtbZIP56_KyoAmpPoolHm-B_ai1
        38.7%
        42%
        11.5
        HYL55DRX3_n02_AtbZIP56_KyoAmpPoolHm-B_ai2
        39.1%
        43%
        9.2
        HYL55DRX3_n02_AtbZIP56_LerAmpPoolHm-B_ai1
        36.4%
        42%
        12.0
        HYL55DRX3_n02_AtbZIP56_LerAmpPoolHm-B_ai2
        35.8%
        43%
        9.5
        HYL55DRX3_n02_AtbZIP56_ShaAmpPoolHm-B_ai1
        35.4%
        41%
        15.9
        HYL55DRX3_n02_AtbZIP56_ShaAmpPoolHm-B_ai2
        35.3%
        42%
        12.6
        HYL55DRX3_n02_AtbZIP57_An1AmpPoolHm-B_ai1
        49.2%
        38%
        5.5
        HYL55DRX3_n02_AtbZIP57_An1AmpPoolHm-B_ai2
        51.4%
        38%
        5.9
        HYL55DRX3_n02_AtbZIP57_C24AmpPoolHm-B_ai1
        49.6%
        37%
        3.1
        HYL55DRX3_n02_AtbZIP57_C24AmpPoolHm-B_ai2
        52.8%
        38%
        3.4
        HYL55DRX3_n02_AtbZIP57_ColAmpPoolHm-B_ai1
        49.9%
        37%
        9.5
        HYL55DRX3_n02_AtbZIP57_ColAmpPoolHm-B_ai2
        51.5%
        38%
        9.8
        HYL55DRX3_n02_AtbZIP57_CviAmpPoolHm-B_ai1
        49.0%
        37%
        5.0
        HYL55DRX3_n02_AtbZIP57_CviAmpPoolHm-B_ai2
        51.2%
        38%
        5.3
        HYL55DRX3_n02_AtbZIP57_EriAmpPoolHm-B_ai1
        49.5%
        37%
        3.9
        HYL55DRX3_n02_AtbZIP57_EriAmpPoolHm-B_ai2
        51.8%
        38%
        4.2
        HYL55DRX3_n02_AtbZIP57_KyoAmpPoolHm-B_ai1
        48.3%
        38%
        6.1
        HYL55DRX3_n02_AtbZIP57_KyoAmpPoolHm-B_ai2
        50.4%
        38%
        6.4
        HYL55DRX3_n02_AtbZIP57_LerAmpPoolHm-B_ai1
        49.8%
        37%
        4.7
        HYL55DRX3_n02_AtbZIP57_LerAmpPoolHm-B_ai2
        52.3%
        38%
        5.0
        HYL55DRX3_n02_AtbZIP57_ShaAmpPoolHm-B_ai1
        49.7%
        37%
        3.5
        HYL55DRX3_n02_AtbZIP57_ShaAmpPoolHm-B_ai2
        53.0%
        38%
        3.9
        HYL55DRX3_n02_AtbZIP68_An1AmpPoolHm-B_ai1
        46.0%
        38%
        4.3
        HYL55DRX3_n02_AtbZIP68_An1AmpPoolHm-B_ai2
        37.1%
        38%
        2.0
        HYL55DRX3_n02_AtbZIP68_C24AmpPoolHm-B_ai1
        49.1%
        37%
        3.1
        HYL55DRX3_n02_AtbZIP68_C24AmpPoolHm-B_ai2
        38.9%
        37%
        1.3
        HYL55DRX3_n02_AtbZIP68_ColAmpPoolHm-B_ai1
        44.6%
        37%
        6.6
        HYL55DRX3_n02_AtbZIP68_ColAmpPoolHm-B_ai2
        37.0%
        38%
        3.4
        HYL55DRX3_n02_AtbZIP68_CviAmpPoolHm-B_ai1
        46.3%
        37%
        4.0
        HYL55DRX3_n02_AtbZIP68_CviAmpPoolHm-B_ai2
        37.1%
        37%
        1.9
        HYL55DRX3_n02_AtbZIP68_EriAmpPoolHm-B_ai1
        47.6%
        37%
        3.5
        HYL55DRX3_n02_AtbZIP68_EriAmpPoolHm-B_ai2
        37.2%
        37%
        1.5
        HYL55DRX3_n02_AtbZIP68_KyoAmpPoolHm-B_ai1
        44.4%
        38%
        4.4
        HYL55DRX3_n02_AtbZIP68_KyoAmpPoolHm-B_ai2
        35.8%
        38%
        2.2
        HYL55DRX3_n02_AtbZIP68_LerAmpPoolHm-B_ai1
        46.8%
        37%
        3.9
        HYL55DRX3_n02_AtbZIP68_LerAmpPoolHm-B_ai2
        37.1%
        38%
        1.8
        HYL55DRX3_n02_AtbZIP68_ShaAmpPoolHm-B_ai1
        48.6%
        37%
        3.3
        HYL55DRX3_n02_AtbZIP68_ShaAmpPoolHm-B_ai2
        38.8%
        38%
        1.4
        HYL55DRX3_n02_PdaTF1_Pda-B_ai1
        64.3%
        38%
        42.7
        HYL55DRX3_n02_PdaTF1_Pda-B_ai2
        69.3%
        37%
        14.1
        HYL55DRX3_n02_PdaTF2_Pda-B_ai1
        62.2%
        38%
        32.7
        HYL55DRX3_n02_PdaTF2_Pda-B_ai2
        59.7%
        38%
        23.7
        HYL55DRX3_n02_pIXHALO_An1AmpPoolHm-B_ai
        26.2%
        38%
        3.3
        HYL55DRX3_n02_pIXHALO_C24AmpPoolHm-B_ai
        26.1%
        37%
        3.6
        HYL55DRX3_n02_pIXHALO_ColAmpPoolHm-B_ai
        26.1%
        38%
        3.5
        HYL55DRX3_n02_pIXHALO_CviAmpPoolHm-B_ai
        27.0%
        37%
        3.7
        HYL55DRX3_n02_pIXHALO_EriAmpPoolHm-B_ai
        21.7%
        36%
        3.0
        HYL55DRX3_n02_pIXHALO_KyoAmpPoolHm-B_ai
        26.9%
        38%
        3.6
        HYL55DRX3_n02_pIXHALO_LerAmpPoolHm-B_ai
        29.0%
        37%
        4.3
        HYL55DRX3_n02_pIXHALO_Pda-B_ai1
        30.6%
        42%
        5.2
        HYL55DRX3_n02_pIXHALO_Pda-B_ai2
        27.4%
        41%
        5.5
        HYL55DRX3_n02_pIXHALO_ShaAmpPoolHm-B_ai
        22.2%
        37%
        3.4
        HYL55DRX3_n02_undetermined
        71.5%
        43%
        96.8

        Demultiplexing Report


        Total Read Count: Total number of PF (Passing Filter) reads in this library.
        Portion: The proportion of reads that represent the individual library in the entire Library Pool.

        Showing 136/136 rows and 2/2 columns.
        LibraryTotal Read CountPortion (%)
        undetermined_library
        96782997
        10.8
        AtbZIP45_ColAmpPoolHm-B_ai1
        10096962
        1.1
        AtbZIP45_C24AmpPoolHm-B_ai1
        3502347
        0.4
        AtbZIP45_EriAmpPoolHm-B_ai1
        4371279
        0.5
        AtbZIP45_CviAmpPoolHm-B_ai1
        5538202
        0.6
        AtbZIP45_ShaAmpPoolHm-B_ai1
        3996874
        0.4
        AtbZIP45_KyoAmpPoolHm-B_ai1
        6477235
        0.7
        AtbZIP45_LerAmpPoolHm-B_ai1
        5154990
        0.6
        AtbZIP45_An1AmpPoolHm-B_ai1
        5889911
        0.7
        AtbZIP45_ColAmpPoolHm-B_ai2
        6683970
        0.7
        AtbZIP45_C24AmpPoolHm-B_ai2
        2361555
        0.3
        AtbZIP45_EriAmpPoolHm-B_ai2
        2943586
        0.3
        AtbZIP45_CviAmpPoolHm-B_ai2
        3734134
        0.4
        AtbZIP45_ShaAmpPoolHm-B_ai2
        2688472
        0.3
        AtbZIP45_KyoAmpPoolHm-B_ai2
        4329555
        0.5
        AtbZIP45_LerAmpPoolHm-B_ai2
        3427796
        0.4
        AtbZIP45_An1AmpPoolHm-B_ai2
        3953014
        0.4
        AtbZIP57_ColAmpPoolHm-B_ai1
        9467201
        1.1
        AtbZIP57_C24AmpPoolHm-B_ai1
        3147409
        0.4
        AtbZIP57_EriAmpPoolHm-B_ai1
        3891921
        0.4
        AtbZIP57_CviAmpPoolHm-B_ai1
        5003586
        0.6
        AtbZIP57_ShaAmpPoolHm-B_ai1
        3529850
        0.4
        AtbZIP57_KyoAmpPoolHm-B_ai1
        6078707
        0.7
        AtbZIP57_LerAmpPoolHm-B_ai1
        4664781
        0.5
        AtbZIP57_An1AmpPoolHm-B_ai1
        5463689
        0.6
        AtbZIP57_ColAmpPoolHm-B_ai2
        9802408
        1.1
        AtbZIP57_C24AmpPoolHm-B_ai2
        3405563
        0.4
        AtbZIP57_EriAmpPoolHm-B_ai2
        4189922
        0.5
        AtbZIP57_CviAmpPoolHm-B_ai2
        5326830
        0.6
        AtbZIP57_ShaAmpPoolHm-B_ai2
        3903421
        0.4
        AtbZIP57_KyoAmpPoolHm-B_ai2
        6362189
        0.7
        AtbZIP57_LerAmpPoolHm-B_ai2
        5043415
        0.6
        AtbZIP57_An1AmpPoolHm-B_ai2
        5855313
        0.7
        AtbZIP68_ColAmpPoolHm-B_ai1
        6570490
        0.7
        AtbZIP68_C24AmpPoolHm-B_ai1
        3082635
        0.3
        AtbZIP68_EriAmpPoolHm-B_ai1
        3492843
        0.4
        AtbZIP68_CviAmpPoolHm-B_ai1
        3995916
        0.4
        AtbZIP68_ShaAmpPoolHm-B_ai1
        3265774
        0.4
        AtbZIP68_KyoAmpPoolHm-B_ai1
        4446992
        0.5
        AtbZIP68_LerAmpPoolHm-B_ai1
        3862427
        0.4
        AtbZIP68_An1AmpPoolHm-B_ai1
        4318792
        0.5
        AtbZIP68_ColAmpPoolHm-B_ai2
        3386295
        0.4
        AtbZIP68_C24AmpPoolHm-B_ai2
        1264615
        0.1
        AtbZIP68_EriAmpPoolHm-B_ai2
        1481843
        0.2
        AtbZIP68_CviAmpPoolHm-B_ai2
        1852686
        0.2
        AtbZIP68_ShaAmpPoolHm-B_ai2
        1364309
        0.2
        AtbZIP68_KyoAmpPoolHm-B_ai2
        2206007
        0.2
        AtbZIP68_LerAmpPoolHm-B_ai2
        1754704
        0.2
        AtbZIP68_An1AmpPoolHm-B_ai2
        2029316
        0.2
        AtbZIP12_ColAmpPoolHm-B_ai1
        8951081
        1.0
        AtbZIP12_C24AmpPoolHm-B_ai1
        2189687
        0.2
        AtbZIP12_EriAmpPoolHm-B_ai1
        3351720
        0.4
        AtbZIP12_CviAmpPoolHm-B_ai1
        5904078
        0.7
        AtbZIP12_ShaAmpPoolHm-B_ai1
        3649694
        0.4
        AtbZIP12_KyoAmpPoolHm-B_ai1
        4766696
        0.5
        AtbZIP12_LerAmpPoolHm-B_ai1
        4043189
        0.5
        AtbZIP12_An1AmpPoolHm-B_ai1
        5842631
        0.7
        AtbZIP12_ColAmpPoolHm-B_ai2
        5659163
        0.6
        AtbZIP12_C24AmpPoolHm-B_ai2
        1346889
        0.1
        AtbZIP12_EriAmpPoolHm-B_ai2
        2046955
        0.2
        AtbZIP12_CviAmpPoolHm-B_ai2
        3540769
        0.4
        AtbZIP12_ShaAmpPoolHm-B_ai2
        2180968
        0.2
        AtbZIP12_KyoAmpPoolHm-B_ai2
        2990134
        0.3
        AtbZIP12_LerAmpPoolHm-B_ai2
        2546401
        0.3
        AtbZIP12_An1AmpPoolHm-B_ai2
        3606901
        0.4
        AtbZIP56_ColAmpPoolHm-B_ai1
        15445599
        1.7
        AtbZIP56_C24AmpPoolHm-B_ai1
        14116848
        1.6
        AtbZIP56_EriAmpPoolHm-B_ai1
        10525302
        1.2
        AtbZIP56_CviAmpPoolHm-B_ai1
        12623152
        1.4
        AtbZIP56_ShaAmpPoolHm-B_ai1
        15877696
        1.8
        AtbZIP56_KyoAmpPoolHm-B_ai1
        11519126
        1.3
        AtbZIP56_LerAmpPoolHm-B_ai1
        12026172
        1.3
        AtbZIP56_An1AmpPoolHm-B_ai1
        11687294
        1.3
        AtbZIP56_ColAmpPoolHm-B_ai2
        12285393
        1.4
        AtbZIP56_C24AmpPoolHm-B_ai2
        11309904
        1.3
        AtbZIP56_EriAmpPoolHm-B_ai2
        8137220
        0.9
        AtbZIP56_CviAmpPoolHm-B_ai2
        10065558
        1.1
        AtbZIP56_ShaAmpPoolHm-B_ai2
        12635216
        1.4
        AtbZIP56_KyoAmpPoolHm-B_ai2
        9210982
        1.0
        AtbZIP56_LerAmpPoolHm-B_ai2
        9478936
        1.1
        AtbZIP56_An1AmpPoolHm-B_ai2
        9341360
        1.0
        AtbZIP20_ColAmpPoolHm-B_ai1
        7818686
        0.9
        AtbZIP20_C24AmpPoolHm-B_ai1
        2296428
        0.3
        AtbZIP20_EriAmpPoolHm-B_ai1
        2932766
        0.3
        AtbZIP20_CviAmpPoolHm-B_ai1
        3927697
        0.4
        AtbZIP20_ShaAmpPoolHm-B_ai1
        2603046
        0.3
        AtbZIP20_KyoAmpPoolHm-B_ai1
        4995063
        0.6
        AtbZIP20_LerAmpPoolHm-B_ai1
        3699885
        0.4
        AtbZIP20_An1AmpPoolHm-B_ai1
        4402739
        0.5
        AtbZIP20_ColAmpPoolHm-B_ai2
        7726705
        0.9
        AtbZIP20_C24AmpPoolHm-B_ai2
        2510408
        0.3
        AtbZIP20_EriAmpPoolHm-B_ai2
        3036529
        0.3
        AtbZIP20_CviAmpPoolHm-B_ai2
        4002220
        0.4
        AtbZIP20_ShaAmpPoolHm-B_ai2
        2770102
        0.3
        AtbZIP20_KyoAmpPoolHm-B_ai2
        4983906
        0.6
        AtbZIP20_LerAmpPoolHm-B_ai2
        3812662
        0.4
        AtbZIP20_An1AmpPoolHm-B_ai2
        4474584
        0.5
        AtbZIP53_ColAmpPoolHm-B_ai1
        3922085
        0.4
        AtbZIP53_C24AmpPoolHm-B_ai1
        5042980
        0.6
        AtbZIP53_EriAmpPoolHm-B_ai1
        3596500
        0.4
        AtbZIP53_CviAmpPoolHm-B_ai1
        4314141
        0.5
        AtbZIP53_ShaAmpPoolHm-B_ai1
        4073846
        0.5
        AtbZIP53_KyoAmpPoolHm-B_ai1
        4019715
        0.4
        AtbZIP53_LerAmpPoolHm-B_ai1
        5079951
        0.6
        AtbZIP53_An1AmpPoolHm-B_ai1
        3732410
        0.4
        AtbZIP53_ColAmpPoolHm-B_ai2
        4536051
        0.5
        AtbZIP53_C24AmpPoolHm-B_ai2
        4150212
        0.5
        AtbZIP53_EriAmpPoolHm-B_ai2
        3761248
        0.4
        AtbZIP53_CviAmpPoolHm-B_ai2
        4721520
        0.5
        AtbZIP53_ShaAmpPoolHm-B_ai2
        4214133
        0.5
        AtbZIP53_KyoAmpPoolHm-B_ai2
        4351874
        0.5
        AtbZIP53_LerAmpPoolHm-B_ai2
        5183807
        0.6
        AtbZIP53_An1AmpPoolHm-B_ai2
        4094515
        0.5
        pIXHALO_ColAmpPoolHm-B_ai
        3528265
        0.4
        pIXHALO_C24AmpPoolHm-B_ai
        3602268
        0.4
        pIXHALO_EriAmpPoolHm-B_ai
        2986104
        0.3
        pIXHALO_CviAmpPoolHm-B_ai
        3679692
        0.4
        pIXHALO_ShaAmpPoolHm-B_ai
        3362736
        0.4
        pIXHALO_KyoAmpPoolHm-B_ai
        3646952
        0.4
        pIXHALO_LerAmpPoolHm-B_ai
        4317700
        0.5
        pIXHALO_An1AmpPoolHm-B_ai
        3282207
        0.4
        AtbZIP26_ColAmpPoolHm-B_ai
        6094706
        0.7
        AtbZIP26_C24AmpPoolHm-B_ai
        8963587
        1.0
        AtbZIP26_EriAmpPoolHm-B_ai
        5656539
        0.6
        AtbZIP26_CviAmpPoolHm-B_ai
        6910212
        0.8
        AtbZIP26_ShaAmpPoolHm-B_ai
        6955437
        0.8
        AtbZIP26_KyoAmpPoolHm-B_ai
        6473316
        0.7
        AtbZIP26_LerAmpPoolHm-B_ai
        8247645
        0.9
        AtbZIP26_An1AmpPoolHm-B_ai
        6044315
        0.7
        PdaTF1_Pda-B_ai1
        42714356
        4.8
        PdaTF1_Pda-B_ai2
        14075739
        1.6
        PdaTF2_Pda-B_ai1
        32722622
        3.6
        PdaTF2_Pda-B_ai2
        23722455
        2.6
        pIXHALO_Pda-B_ai1
        5215539
        0.6
        pIXHALO_Pda-B_ai2
        5537881
        0.6
        AtTGA5_Col-B_aiqiagen
        608359
        0.1

        Barcodes of Undetermined Reads


        We have determined the barcodes of your undetermined reads. Here are the top 20 barcodes. The full list is available here. If your libraries are dual indexed, the two indices are concatenated.

        Showing 20/20 rows and 2/2 columns.
        Barcode Sequence(s)CountFrequency (%)
        GGGGGGGGAGATCTCG
        63212724.0
        65.3
        GGGGGGGGGTCAGTAC
        2013457.0
        2.1
        GGGGGGGGTAAGATTA
        955921.0
        1.0
        GGGGGGGGCGTGTGAT
        741067.0
        0.8
        GGGGGGGGTAACGTCG
        536624.0
        0.6
        TCTCGCGCGGGGGGGG
        370113.0
        0.4
        GGGGGGGGAGGCTATA
        327284.0
        0.3
        GGGGGGGGCGATCTCG
        302317.0
        0.3
        CGGCTAATGGGGGGGG
        283146.0
        0.3
        GGGGGGGGAGTTCTCG
        266965.0
        0.3
        GGGGGGGGAGGATAGG
        261010.0
        0.3
        GGGGGGGGGCCTCTAT
        231431.0
        0.2
        GGGGGGGGAAGGCGTA
        231296.0
        0.2
        GGGGGGGGTGGAAGCA
        212280.0
        0.2
        GGGGGGGGAACCTCTC
        203731.0
        0.2
        TGGACTCTGGGGGGGG
        193357.0
        0.2
        ATCCAGAGGGGGGGGG
        185692.0
        0.2
        GCTATCCTGGGGGGGG
        182926.0
        0.2
        AGAGTAGCGGGGGGGG
        171836.0
        0.2
        GGGGGGGGCTTCGCCT
        170688.0
        0.2

        Run Statistics

        Showing 1/1 rows and 4/4 columns.
        Number of LanesTotal # of Single-End ReadsTotal # PF Reads% Undetermined% PhiX Aligned
        2.0
        1276674048
        897484488
        10.8
        7.1

        FastQC

        FastQC is a quality control tool for high throughput sequence data, written by Simon Andrews at the Babraham Institute in Cambridge.

        Sequence Counts

        Sequence counts for each sample. Duplicate read counts are an estimate only.

        This plot show the total number of reads, broken down into unique and duplicate if possible (only more recent versions of FastQC give duplicate info).

        You can read more about duplicate calculation in the FastQC documentation. A small part has been copied here for convenience:

        Only sequences which first appear in the first 100,000 sequences in each file are analysed. This should be enough to get a good impression for the duplication levels in the whole file. Each sequence is tracked to the end of the file to give a representative count of the overall duplication level.

        The duplication detection requires an exact sequence match over the whole length of the sequence. Any reads over 75bp in length are truncated to 50bp for this analysis.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Sequence Quality Histograms

        The mean quality value across each base position in the read.

        To enable multiple samples to be plotted on the same graph, only the mean quality scores are plotted (unlike the box plots seen in FastQC reports).

        Taken from the FastQC help:

        The y-axis on the graph shows the quality scores. The higher the score, the better the base call. The background of the graph divides the y axis into very good quality calls (green), calls of reasonable quality (orange), and calls of poor quality (red). The quality of calls on most platforms will degrade as the run progresses, so it is common to see base calls falling into the orange area towards the end of a read.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Per Sequence Quality Scores

        The number of reads with average quality scores. Shows if a subset of reads has poor quality.

        From the FastQC help:

        The per sequence quality score report allows you to see if a subset of your sequences have universally low quality values. It is often the case that a subset of sequences will have universally poor quality, however these should represent only a small percentage of the total sequences.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Per Base Sequence Content

        The proportion of each base position for which each of the four normal DNA bases has been called.

        To enable multiple samples to be shown in a single plot, the base composition data is shown as a heatmap. The colours represent the balance between the four bases: an even distribution should give an even muddy brown colour. Hover over the plot to see the percentage of the four bases under the cursor.

        To see the data as a line plot, as in the original FastQC graph, click on a sample track.

        From the FastQC help:

        Per Base Sequence Content plots out the proportion of each base position in a file for which each of the four normal DNA bases has been called.

        In a random library you would expect that there would be little to no difference between the different bases of a sequence run, so the lines in this plot should run parallel with each other. The relative amount of each base should reflect the overall amount of these bases in your genome, but in any case they should not be hugely imbalanced from each other.

        It's worth noting that some types of library will always produce biased sequence composition, normally at the start of the read. Libraries produced by priming using random hexamers (including nearly all RNA-Seq libraries) and those which were fragmented using transposases inherit an intrinsic bias in the positions at which reads start. This bias does not concern an absolute sequence, but instead provides enrichement of a number of different K-mers at the 5' end of the reads. Whilst this is a true technical bias, it isn't something which can be corrected by trimming and in most cases doesn't seem to adversely affect the downstream analysis.

        Click a sample row to see a line plot for that dataset.
        Rollover for sample name
        Position: -
        %T: -
        %C: -
        %A: -
        %G: -

        Per Sequence GC Content

        The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content.

        From the FastQC help:

        This module measures the GC content across the whole length of each sequence in a file and compares it to a modelled normal distribution of GC content.

        In a normal random library you would expect to see a roughly normal distribution of GC content where the central peak corresponds to the overall GC content of the underlying genome. Since we don't know the the GC content of the genome the modal GC content is calculated from the observed data and used to build a reference distribution.

        An unusually shaped distribution could indicate a contaminated library or some other kinds of biased subset. A normal distribution which is shifted indicates some systematic bias which is independent of base position. If there is a systematic bias which creates a shifted normal distribution then this won't be flagged as an error by the module since it doesn't know what your genome's GC content should be.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Per Base N Content

        The percentage of base calls at each position for which an N was called.

        From the FastQC help:

        If a sequencer is unable to make a base call with sufficient confidence then it will normally substitute an N rather than a conventional base call. This graph shows the percentage of base calls at each position for which an N was called.

        It's not unusual to see a very low proportion of Ns appearing in a sequence, especially nearer the end of a sequence. However, if this proportion rises above a few percent it suggests that the analysis pipeline was unable to interpret the data well enough to make valid base calls.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Sequence Length Distribution

        All samples have sequences of a single length (101bp).

        Sequence Duplication Levels

        The relative level of duplication found for every sequence.

        From the FastQC Help:

        In a diverse library most sequences will occur only once in the final set. A low level of duplication may indicate a very high level of coverage of the target sequence, but a high level of duplication is more likely to indicate some kind of enrichment bias (eg PCR over amplification). This graph shows the degree of duplication for every sequence in a library: the relative number of sequences with different degrees of duplication.

        Only sequences which first appear in the first 100,000 sequences in each file are analysed. This should be enough to get a good impression for the duplication levels in the whole file. Each sequence is tracked to the end of the file to give a representative count of the overall duplication level.

        The duplication detection requires an exact sequence match over the whole length of the sequence. Any reads over 75bp in length are truncated to 50bp for this analysis.

        In a properly diverse library most sequences should fall into the far left of the plot in both the red and blue lines. A general level of enrichment, indicating broad oversequencing in the library will tend to flatten the lines, lowering the low end and generally raising other categories. More specific enrichments of subsets, or the presence of low complexity contaminants will tend to produce spikes towards the right of the plot.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Overrepresented sequences

        The total amount of overrepresented sequences found in each library.

        FastQC calculates and lists overrepresented sequences in FastQ files. It would not be possible to show this for all samples in a MultiQC report, so instead this plot shows the number of sequences categorized as over represented.

        Sometimes, a single sequence may account for a large number of reads in a dataset. To show this, the bars are split into two: the first shows the overrepresented reads that come from the single most common sequence. The second shows the total count from all remaining overrepresented sequences.

        From the FastQC Help:

        A normal high-throughput library will contain a diverse set of sequences, with no individual sequence making up a tiny fraction of the whole. Finding that a single sequence is very overrepresented in the set either means that it is highly biologically significant, or indicates that the library is contaminated, or not as diverse as you expected.

        FastQC lists all of the sequences which make up more than 0.1% of the total. To conserve memory only sequences which appear in the first 100,000 sequences are tracked to the end of the file. It is therefore possible that a sequence which is overrepresented but doesn't appear at the start of the file for some reason could be missed by this module.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Adapter Content

        The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position.

        Note that only samples with ≥ 0.1% adapter contamination are shown.

        There may be several lines per sample, as one is shown for each adapter detected in the file.

        From the FastQC Help:

        The plot shows a cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position. Once a sequence has been seen in a read it is counted as being present right through to the end of the read so the percentages you see will only increase as the read length goes on.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Status Checks

        Status for each FastQC section showing whether results seem entirely normal (green), slightly abnormal (orange) or very unusual (red).

        FastQC assigns a status for each section of the report. These give a quick evaluation of whether the results of the analysis seem entirely normal (green), slightly abnormal (orange) or very unusual (red).

        It is important to stress that although the analysis results appear to give a pass/fail result, these evaluations must be taken in the context of what you expect from your library. A 'normal' sample as far as FastQC is concerned is random and diverse. Some experiments may be expected to produce libraries which are biased in particular ways. You should treat the summary evaluations therefore as pointers to where you should concentrate your attention and understand why your library may not look random and diverse.

        Specific guidance on how to interpret the output of each module can be found in the relevant report section, or in the FastQC help.

        In this heatmap, we summarise all of these into a single heatmap for a quick overview. Note that not all FastQC sections have plots in MultiQC reports, but all status checks are shown in this heatmap.

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