FastQCFastQC Report
Wed 24 Apr 2024
HYL55DRX3_n01_AtTGA5_Col-B_aiqiagen.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYL55DRX3_n01_AtTGA5_Col-B_aiqiagen.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences608359
Sequences flagged as poor quality0
Sequence length101
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTATGCC15930.26185196569788566TruSeq Adapter, Index 8 (96% over 33bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCGCGTATGCC12190.20037510746121942TruSeq Adapter, Index 8 (96% over 33bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)