FastQCFastQC Report
Sat 9 Mar 2019
HYKTJBGX9_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYKTJBGX9_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35833205
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21096295.887357829141992No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGAC30400.013.0981077
CTAGACT31000.012.0898258
GAGGTCA38750.010.4071852
ATTCTAG40700.010.3704675
CGGAAAT57700.09.9177631
CGGGAAT25400.09.0488211
CCGGGAG41700.08.9987791
CATTCTA48300.08.9320324
TTCTAGA45250.08.9132766
TTCGTAC52400.08.7699983
AGGGGGG53250.08.2801451
TAGACTT47100.08.1568799
GGCATTG47200.07.94790553
CGACGAT79750.07.82259131
CTGGAAT45050.07.8090131
CGTACAA53750.07.6781115
CTCGGAT22900.07.5787211
CGGCTCT31900.07.35205941
GGCTCTC35000.07.23489482
CTCGGGT29400.07.17951061
Produced by FastQC (version 0.11.5)