FastQCFastQC Report
Sat 9 Mar 2019
HYKTJBGX9_n01_WV2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYKTJBGX9_n01_WV2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22407341
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCTACACCCAAAATTATATCTTAATCCAACATCGAGGTCGCAATCTTT291600.13013592286563586No Hit
CTCGTCTTTTAAATAAATTTTAGCTTTTTGACTAAAAAATAAAATTCTAT291010.129872616300167No Hit
CAAAAACATGTCTTTTTGAATTATATATAAAGTCTAACCTGCCCACTGAA259120.1156406732954169No Hit
GAAAATTTTAAATGGCCGCAGTATTTTGACTGTGCAAAGGTAGCATAATC230470.10285468498917386No Hit
GTCCTTTCGTACTAAAATATCACAATTTTTTAAAGATAGAAACCAACCTG226210.10095352233002568No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACTA207250.017.7584048
CCTTTCG211350.017.7515073
GTACTAA216550.017.1053989
TTCGTAC232000.016.150896
GTCCTTT228250.016.0055051
TCCTTTC239750.015.8669682
AGGGGGG45250.015.5380661
TGGGGGG108850.014.9262771
CTTTCGT250150.014.8461784
TTTCGTA274200.013.9943065
CTCGTCT257450.013.5627661
CGCAAAT168600.013.44047451
CGGCTAC242800.013.4028051
TCGTACT296500.013.1817167
GCTACAC239850.013.0681643
TACACCC244200.012.93252855
GGCTACA251500.012.5952822
CGGGTCT60350.011.9652051
CTACACC268000.011.7840694
CGTCTTT275150.011.7713213
Produced by FastQC (version 0.11.5)