FastQCFastQC Report
Sat 9 Mar 2019
HYKTJBGX9_n01_WV1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYKTJBGX9_n01_WV1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34342598
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCTACACCCAAAATTATATCTTAATCCAACATCGAGGTCGCAATCTTT423380.12328129630728578No Hit
CTCGTCTTTTAAATAAATTTTAGCTTTTTGACTAAAAAATAAAATTCTAT362620.10558898310488915No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACTA275400.016.6918038
GTACTAA283650.016.122619
CCTTTCG289150.015.9969933
GTCCTTT302850.015.1693131
TTCGTAC311500.015.0015416
TCCTTTC328150.014.3131532
CTTTCGT337150.013.8038974
AGGGGGG66600.013.6960091
TGGGGGG164350.013.2681391
TTTCGTA376250.012.7480475
CGGCTAC363500.012.2854331
GCTACAC353650.012.0185473
TACACCC352800.011.8724085
CGCAAAT260550.011.8519361
CTCGTCT362950.011.8066511
GGCTACA370050.011.7813132
TCGTACT411150.011.7697277
CGGGTCT94850.011.2696961
GGGGTCT63750.011.2528831
CTACACC394800.010.7417024
Produced by FastQC (version 0.11.5)