FastQCFastQC Report
Sat 9 Mar 2019
HYKTJBGX9_n01_PT9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYKTJBGX9_n01_PT9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19785679
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA67000.016.2315584
TGGGGGG81350.016.0000761
TCTAGAC103050.011.5670637
CTAGACT101000.011.3316468
AGGTCAT86450.011.0415933
ATTCTAG136600.010.3948945
TAATCCC106450.010.3053729
GTCATAA112850.010.0576355
TTCTAGA142750.09.6809156
CGGGAAT105200.08.7602211
GAGGTCA112850.08.7111162
CATTCTA157200.08.60977654
GGGGAAT63650.08.2842591
AGGGGGG59250.08.2580591
TAGACTT146450.08.2040549
CGGGGAT54850.08.14104751
CGGCAAT117400.08.1331181
CGGGCAT48600.08.11280251
GACCCTA45650.07.9062835
CTGGGAT59550.07.81759931
Produced by FastQC (version 0.11.5)