FastQCFastQC Report
Sat 9 Mar 2019
HYKTJBGX9_n01_PT8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYKTJBGX9_n01_PT8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22457472
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG109950.022.6417481
GGTCATA86800.017.3988594
AGGTCAT94650.014.6011713
GAGGTCA96050.014.1413472
TAATCCC121550.012.9323019
AGGGGGG78050.011.260891
GTCATAA139050.011.1000715
CTAGACT138550.010.9385071
TCTAGAC137900.010.4003127
CGGGGAT56900.010.2699131
CCCGGAT45750.010.1767451
GGGGAAT60700.09.9400591
CGAGGTC144600.09.3637651
ATTCTAG172300.09.3163295
TTGGGGG42050.09.2644951
GTATGCC83000.09.1557644-45
TATGCCG105450.08.96347546-47
AGTCACC108300.08.87974128-29
CTGGGAT55550.08.7234851
GACCCTA54150.08.5947127
Produced by FastQC (version 0.11.5)