FastQCFastQC Report
Sat 9 Mar 2019
HYKTJBGX9_n01_PT7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYKTJBGX9_n01_PT7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25467187
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTCGTACAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCT456140.17910890590311368No Hit
CAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCTCACACCGA362750.1424381891883073No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG354970.13938327778407564No Hit
CGAGGTCATAATCCCAATTATATATATACTACAAAATTAAATTATGCTGT260240.10218639380941445No Hit
CTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTAC257110.10095736132930581No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG149100.025.7762581
GGTCATA127500.023.987494
GAGGTCA140700.019.4760592
AGGGGGG73850.018.3977411
AGGTCAT149350.018.1250323
TAATCCC172000.017.7535349
GTCATAA186900.016.4909065
ACTCTAG55900.014.6974985
TCTAGAC196300.014.515577
CTAGACT196950.014.2506228
CGAGGTC197300.013.7004291
TTGGGGG51000.013.3203251
CTAGTTG61500.012.6640048
ATAATCC252000.012.400178
CGTACAA400850.012.3332625
TTCGTAC388350.012.1799393
ATTCTAG256050.012.0743935
ATTATAG258550.011.9392755
TTCTAGA252900.011.8679716
CTTTCGT453000.011.4622781
Produced by FastQC (version 0.11.5)