FastQCFastQC Report
Sat 9 Mar 2019
HYKTJBGX9_n01_PT6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYKTJBGX9_n01_PT6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26989292
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGCATTGCCGTCATCGGCGGTGGTGGTGGAATCGGAGGTGGTGCCCTC374310.1386883361001096No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA71950.017.1610834
CTGGCAT254200.016.8194391
GGCATTG269900.015.2024853
TGGCATT276500.014.9085832
GCATTGC273000.014.7688664
CCACAAT131550.014.7337691
TCTAGAC104450.013.7762387
TGACTAA235100.013.49334058
CTAGACT107600.013.3729148
TTGACTA246500.013.1583147
CTGGATT218200.012.9976281
GACTAAA240000.012.9606189
ATGGCCC118950.012.78522694-95
TGGGGGG119600.012.631081
ATTCGGT213850.012.5248335
ACTGTCC102800.012.2420476
TAATCCC101300.012.1887629
AGGTCAT87800.012.1159433
TTCGGTG217950.011.9841476
GCCGTCA334450.011.827959
Produced by FastQC (version 0.11.5)