FastQCFastQC Report
Sat 9 Mar 2019
HYKTJBGX9_n01_PT5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYKTJBGX9_n01_PT5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31794021
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGCATTGCCGTCATCGGCGGTGGTGGTGGAATCGGAGGTGGTGCCCTC441800.13895694413738985No Hit
CGGAAATTGACTAAATTTTACCCGAGTCACCCGACTGTAACGGACACCCG334990.10536257744813088No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA111200.023.5320644
AGGTCAT127650.018.0441133
TAATCCC146800.017.5987839
GTCATAA150600.017.3440425
CTGGCAT306700.016.9297311
ACTGTCC132800.015.9496096
GGCATTG330750.015.6653283
GCACTAG65900.015.5660419
TGGCATT331950.015.1797332
CTGTCCA139250.015.0060817
GCATTGC339000.014.9898514
CCACAAT155750.014.9150441
TCTAGAC169600.014.8128847
CGGCTCT153650.014.716961
CTAGACT176500.014.2876118
AACTGTC156850.014.0187415
GGCTACC62800.013.9146226
CGCACTA75150.013.8396458
ATAATCC194700.013.5618438
AAGGCTA68050.013.399434
Produced by FastQC (version 0.11.5)