FastQCFastQC Report
Sat 9 Mar 2019
HYKTJBGX9_n01_PT4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYKTJBGX9_n01_PT4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25629566
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAATCGGAGGTGGTGCCCTCGGTGGCGGTGATGCCGGAGTTGCTGTG392040.15296396357238354No Hit
CGAGTATGTAATAATAATATGTATGCTTGGGTGCGGCGGTAAGTATAACA265810.10371225170180406No Hit
CTGGCATTGCCGTCATCGGCGGTGGTGGTGCTGGAATCGGAGGTGGTGCC260270.10155068564173111No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA81850.019.6691234
TAATCCC109550.014.9989239
AGGTCAT99250.014.7375133
GTCATAA117500.014.1055995
TCTAGAC119350.013.7673667
ACTGTCC100600.013.4067756
CTGGAAT349050.013.1874641
CCACAAT112250.012.9497151
CTAGACT126050.012.8471828
CTGTCCA103400.012.7679587
CGGCTCT120050.012.6623111
CGAGTAT264700.012.4007861
GAATCGG366550.012.3988874
GGAATCG374500.012.2117643
GTGTGGC223950.012.1932866
TGACTAA208900.012.1849198
AATCGGA364700.012.0841515
CGGGCAT34300.012.048981
TGGGGGG122000.011.9537371
CTGGCAT272850.011.7692251
Produced by FastQC (version 0.11.5)