FastQCFastQC Report
Sat 9 Mar 2019
HYKTJBGX9_n01_PT3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYKTJBGX9_n01_PT3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14400642
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG213410.14819478187153046No Hit
GTTAAAAGAAAAGAAACTTTAATAAATATTTTTTCCATAGATGAATGATT180390.1252652485910003No Hit
CGAGGTCATAATCCCAATTATATATATACTACAAAATTAAATTATGCTGT160960.11177279457401969No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG155330.10786324665247563No Hit
CTGGCATTGCCGTCATCGGCGGTGGTGGTGGAATCGGAGGTGGTGCCCTC144970.10066912294604644No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA76500.025.8250924
AGGTCAT89500.020.1638583
GTCATAA99600.020.121565
TGGGGGG126000.019.4175531
TAATCCC130800.015.6484749
ATAATCC130850.015.2069748
GAGGTCA147050.014.921092
CGCACTA29100.014.6876398
CTGGCAT112200.014.56543351
GGGTTCG56050.014.1498184
GGATAGT72900.014.0761561
GCGTACC31200.014.0041522
TCGGGTT57100.013.9732282
CGGCTCT56750.013.8963281
GTTCGGG55900.013.6786
GCACTAG31950.013.5261139
ACTCTAG33450.013.4876415
GGTTCGG56700.013.4850125
CGGGTTC58950.013.4538223
CGAGTAT103400.013.1402421
Produced by FastQC (version 0.11.5)