FastQCFastQC Report
Sat 9 Mar 2019
HYKTJBGX9_n01_PT2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYKTJBGX9_n01_PT2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28384352
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGCATTGCCGTCATCGGCGGTGGTGGTGGAATCGGAGGTGGTGCCCTC436200.15367622273004508No Hit
CGGAAATTGACTAAATTTTACCCGAGTCACCCGACTGTAACGGACACCCG324640.11437287699927058No Hit
CGAGTATGTAATAATAATATGTATGCTTGGGTGCGGCGGTAAGTATAACA292400.10301450602078215No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA103300.025.3316234
AGGTCAT114700.020.7851723
CTGGCAT289050.019.2782171
GTCATAA140650.019.2462845
TAATCCC143850.018.5208179
TGGCATT314850.017.3615912
GGCATTG334600.017.2308243
GCATTGC345000.016.4497994
TCTAGAC180700.016.4258967
CCACAAT154750.015.9323021
GCACTAG56250.015.8723959
CGGCTCT127550.015.3819511
CTAGACT196150.015.0594558
GCGTACC66000.014.9670862
TGGGGGG148900.014.7397171
ATTCTAG213850.014.4793835
TGCCGTC394700.014.233938
ATAATCC191150.014.01238258
TTCTAGA220200.013.9755436
ATGGCCC126000.013.95517494-95
Produced by FastQC (version 0.11.5)