FastQCFastQC Report
Sat 9 Mar 2019
HYKTJBGX9_n01_PT1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYKTJBGX9_n01_PT1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19361987
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTAC332050.17149582839819075No Hit
CTTTCGTACAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCT320760.16566481528987703No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG301610.15577430147019516No Hit
CGAGGTCATAATCCCAATTATATATATACTACAAAATTAAATTATGCTGT294840.15227775950887684No Hit
GTTAAAAGAAAAGAAACTTTAATAAATATTTTTTCCATAGATGAATGATT271240.14008892785642302No Hit
CTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTACAGTCAAT250110.129175791720137No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG237700.12276632558424919No Hit
CAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCTTACACCGA232270.11996186135234985No Hit
CTCCTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTG221010.11414634252156042No Hit
GTTTAATAAAAACATTGTTTTTAGAGTATAATTTAAAATTAGTTCTGCTC204490.10561416036484272No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA109050.031.1378654
AGGTCAT124300.025.1782843
GTCATAA149050.023.0682725
TAATCCC153750.022.0229249
GAGGTCA158450.020.8909932
TGGGGGG166350.020.3048571
ACTCTAG45800.019.079235
ATAATCC189650.018.2296838
CTAGTTG45650.017.4770898
TCTAGAC210850.016.5995247
CTAGACT227800.015.1975798
CTCTAGT59100.014.9462696
TCATAAT239950.014.6657666
CGGCTCT108800.014.5838011
CCGGGAG168100.014.5543461
CTGGCAT91750.014.5496791
TTCTAGA248450.014.4316266
ATTCTAG245100.014.4157785
ATTATAG172450.014.4028015
CGAGGTC235500.014.1410051
Produced by FastQC (version 0.11.5)