FastQCFastQC Report
Sat 9 Mar 2019
HYKTJBGX9_n01_PT17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYKTJBGX9_n01_PT17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28530097
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG310640.10888150853465377No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG142600.023.8203221
GGTCATA127600.021.2890974
GAGGTCA130600.018.5096042
AGGTCAT132150.018.220183
TAATCCC169900.016.2959699
GTCATAA187350.014.8544235
TCTAGAC200500.014.5905667
CTAGACT193300.014.5689378
CGAGGTC176150.013.9703791
ATTCTAG256750.012.30050855
AGGGGGG76050.011.9939741
TTCTAGA262500.011.5968246
TAGACTT251550.011.5728739
ATAATCC259300.011.2452968
CATTCTA283800.011.1448364
TTGGGGG54450.010.8189441
CCCAGTT66450.010.438041
CGGGGAT60400.010.0677891
CCCAATT202800.09.3000151
GTTTAAT349850.09.1524871
Produced by FastQC (version 0.11.5)