FastQCFastQC Report
Sat 9 Mar 2019
HYKTJBGX9_n01_PT16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYKTJBGX9_n01_PT16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31516535
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG340700.10810198519602487No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA121550.023.9503974
TGGGGGG195400.019.0846671
AGGTCAT147050.017.6656723
TAATCCC171150.017.453169
GTCATAA174950.016.8300325
TCTAGAC216500.015.6398277
CTGGCAT194900.015.5750181
CTAGACT217450.015.4841418
GGCATTG208950.014.09153
CGGCTCT146650.013.8644041
ATTCTAG265750.013.7423275
ATAATCC223900.013.6169948
TTCTAGA269850.013.2695686
GCATTGC219050.013.2466224
TAGACTT262850.012.95422659
TGGCATT229450.012.77061752
GAGGTCA252650.012.5941912
CATTCTA294350.012.5845524
TCCGCAT167600.012.3541487
CGAGTAT275000.012.1958341
Produced by FastQC (version 0.11.5)