FastQCFastQC Report
Sat 9 Mar 2019
HYKTJBGX9_n01_PT12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYKTJBGX9_n01_PT12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26928627
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG110550.020.4561
GGTCATA106850.019.5565644
AGGTCAT115100.016.1331163
CTAGACT172050.014.8503398
TAATCCC140150.014.8080239
TCTAGAC174400.014.7591867
GAGGTCA135200.014.1212082
GTCATAA156050.013.6341695
CCACCAG205300.012.5608688
ATTCTAG220200.012.4659385
GACCAGC188850.012.3728752
CGACCAG199000.012.1278311
TTCTAGA227300.011.7840386
CATTCTA250550.011.7140884
ACCAGCC205100.011.6702923
TAGACTT225950.011.5179989
CCCAGTT67950.011.4663941
AGCCACC221350.011.3927736
CGAGGTC182850.010.7826541
AGGGGGG65150.010.0632251
Produced by FastQC (version 0.11.5)