FastQCFastQC Report
Sat 9 Mar 2019
HYKTJBGX9_n01_PT11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYKTJBGX9_n01_PT11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33371229
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG155350.020.9467051
GGTCATA142650.019.175814
AGGTCAT153250.015.8352643
TCTAGAC238200.015.1519417
GAGGTCA160700.015.1307912
CTAGACT235950.014.9542738
TAATCCC195100.014.1421469
GTCATAA202950.013.712345
ATTCTAG301300.012.6251455
TAGACTT303000.011.9742189
TTCTAGA311850.011.92396
CATTCTA335350.011.6972754
AGGGGGG88100.011.5930821
CCACCAG238700.011.0616238
ACCAGCC224600.011.0162283
CGAGGTC220250.010.84896851
CGACCAG254150.010.4299081
AGCCACC242300.010.4270296
GACCAGC244550.010.40889552
TTGGGGG61350.010.0661911
Produced by FastQC (version 0.11.5)