FastQCFastQC Report
Sat 9 Mar 2019
HYKTJBGX9_n01_PT10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYKTJBGX9_n01_PT10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28259275
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG127300.023.3239151
GGTCATA117400.020.1451824
GAGGTCA131500.016.2883722
AGGTCAT129850.016.2753773
TAATCCC160900.014.8755999
TCTAGAC193050.014.7352317
CTAGACT189100.014.6663258
GTCATAA175700.013.4877015
CGAGGTC175750.012.3799811
AGGGGGG72650.012.0972311
ATTCTAG248050.012.0617555
TTCTAGA257450.011.4368716
CATTCTA269350.011.3724154
TAGACTT251100.011.3097889
CGGGGAT64100.010.6722081
ATAATCC241500.010.0485578
TTGGGGG49550.09.9710191
CCCAGTT70450.09.1708121
CGGGAAT131650.08.9491261
AGCCACC144750.08.6615136
Produced by FastQC (version 0.11.5)